Browsing by Subject "Liver regeneration"

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Open Access
Administration of bone marrow derived mesenchymal stem cells modulate tlr expression during liver regeneration
(Trakya Üniversitesi, 2019) Koçak, H.; Tokçaer Keskin, Z.; İnsal, B.; Gürsel, İhsan; Akçalı, K. C.
Liver cell transplantation is a powerful alternative to orthotopic cell transplantation in the treatment of liver failures. Recently, considerable effort is being channeled to understand the nature and kinetics of directing stem cells to effectively accumulate at the regenerating liver site. Mesenchymal stem cells are one of the promising cell sources modulating liver regeneration process. The present was designed to study how mesenchymal stem cells might modulate liver immune behaviors by changing Toll-like receptor (TLR) expression and increase regenerative potential during liver regeneration in rats. Normal and partially hepatectomized rats were treated with mesenchymal stem cells isolated and expanded from rat bone marrows. Accumulation of mesenchymal stem cells was confirmed by Real Time-Polymerase Chain Reaction (RT-PCR), Fluorescence-Activated Cell Sorting (FACS), and Immunofluorescence Staining (IFS). Student's t-test analysis was used to evaluate the significance of differences between sham and partially hepatectomized rat groups. Our results showed that mesenchymal stem cells expressed several TLRs, and their accumulation during regeneration was depended on the timing of injury. Mesenchymal stem cells isolated from bone marrow of normal rats were observed at the injured liver 3 days after the injection. There were no labeled mesenchymal stem cells in the liver sections of the uninjured animals. Mesenchymal stem cell administration significantly altered the expression of TLR2, 3 and 9 while retaining their migration potential to regenerating liver. Our findings implicated that mesenchymal stem cell administration during liver regeneration modulate the immune response through changing the expression of the TLRs in the remaining liver parts into which the cells are recruited or infused. This alteration may contribute to the regeneration process following partial hepatectomy.
Open Access
Cloning and expression profile of FLT3 gene during progenitor cell-dependent liver regeneration
(Blackwell Publishing, 2007) Aydin, I. T.; Tokcaer, Z.; Dalgic, A.; Konu, O.; Akcali, K. C.
Background and Aim: The liver has a unique capacity to regenerate upon exposure to viral infections, toxic reactions and cancer formation. Liver regeneration is a complex phenomenon in which several factors participate during its onset. Cellular proliferation is an important component of this process and the factors that regulate this proliferation have a vital role. FLT3, a well-known hematopoietic stem cell and hepatic lineage surface marker, is involved in proliferative events of hematopoietic stem cells. However, its contribution to liver regeneration is not known. Therefore, the aim of this study was to clone and examine the role of FLT3 during liver regeneration in rats. Methods: Partial cDNA of rat homolog of FLT3 gene was cloned from thymus and the tissue specific expression of this gene at mRNA and protein levels was examined by RT-PCR and Western blot. After treating with 2-AAF and performing hepatectomy in rats to induce progenitor-dependent liver regeneration, the mRNA and protein expression profile of FLT3 was investigated by real-time PCR and Western blot during liver regeneration. In addition, cellular localization of FLT3 protein was determined by immunohistochemistry. Results: The results indicated that rat FLT3 cDNA has high homology with mouse and human FLT3 cDNA. It was also found that FLT3 is expressed in most of the rat tissues and during liver regeneration. In addition, its intracellular localization is altered during the late stages of liver regeneration. Conclusion: The FLT3 receptor is activated at the late stages of liver regeneration and participates in the proliferation response that is observed during progenitor-dependent liver regeneration. © 2006 The Authors.
Open Access
Cloning and expression profile of FLT3 gene during rat liver regeneration
(2005) Aydın, Iraz Toprak
Liver has a unique capacity to regenerate itself upon exposure to viral infections, toxic reactions and cancer formation which result in the loss of hepatocyte. Liver regeneration is a complex phenomenon in which several factors participate during its onset. Cellular proliferation is one of the important component of this process and the factors regulate this proliferation has a vital role. FLT3, a well-known hematopoietic stem cell and hepatic lineage surface marker, takes place in proliferative events of hematopoietic stem cells. However, its contribution to liver regeneration is not known. Therefore, in this study, we aimed to examine the role of FLT3 during liver regeneration. First we cloned a partial cDNA of rat homolog of FLT3 from thymus and examined the tissue specific expressions both in mRNA and protein level. After performing hepatectomy in rats to induce liver regeneration, expression profile of FLT3 in both mRNA and protein level and its cellular localization were also investigated during the different stages of liver regeneration models. Our data showed that FLT3 is expressed in most of the rat tissues and in liver regeneration. However, its intracellular localization is changed during the late stages of liver regeneration. Therefore, our results suggest a mechanism in which FLT3 receptor is activated in the late stages of liver regeneration.