Browsing by Subject "Hybrid nanoparticles"

Now showing 1 - 3 of 3

Open Access
Farnesylthiosalicylic acid-loaded lipid–polyethylene glycol–polymer hybrid nanoparticles for treatment of glioblastoma
(Blackwell Publishing Ltd, 2017) Kaffashi, A.; Lüle, S.; Pehlivan, S. B.; Sarısözen, C.; Vural, İ.; Koşucu, H.; Demir, T.; Buğdaycı, K. E.; Söylemezoğlu, F.; Oğuz, K. K.; Mut, M.
Objectives: We aimed to develop lipid–polyethylene glycol (PEG)–polymer hybrid nanoparticles, which have high affinity to tumour tissue with active ingredient, a new generation antineoplastic drug, farnesylthiosalicylic acid (FTA) for treatment of glioblastoma. Method: Farnesylthiosalicylic acid-loaded poly(lactic-co-glycolic acid)-1,2 distearoyl-glycerol-3-phospho-ethanolamine-N [methoxy (PEG)-2000] ammonium salt (PLGA-DSPE-PEG) with or without 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) hybrid nanoparticles has been prepared and evaluated for in-vitro characterization. Cytotoxicity of FTA-loaded nanoparticles along with its efficacy on rat glioma-2 (RG2) cells was also evaluated both in vitro (in comparison with non-malignant cell line, L929) and in vivo. Key findings: Scanning electron microscopy studies showed that all formulations prepared had smooth surface and spherical in shape. FTA and FTA-loaded nanoparticles have cytotoxic activity against RG2 glioma cell lines in cell culture studies, which further increases with addition of DOTAP. Magnetic resonance imaging and histopathologic evaluation on RG2 tumour cells in rat glioma model (49 female Wistar rats, 250–300 g) comparing intravenous and intratumoral injections of the drug have been performed and FTA-loaded nanoparticles reduced tumour size significantly in in-vivo studies, with higher efficiency of intratumoral administration than intravenous route. Conclusion: Farnesylthiosalicylic acid-loaded PLGA-DSPE-PEG-DOTAP hybrid nanoparticles are proven to be effective against glioblastoma in both in-vitro and in-vivo experiments. © 2017 Royal Pharmaceutical Society
Open Access
Multi-step microfludic reactor for the synthesis of hybrid nanoparticles
(Institute of Physics Publishing, 2020) Wahab, Malik Abdul; Erdem, E. Yegan
We introduce a multi-step microfluidic reactor for the synthesis of hybrid nanoparticles. As part of this study, nanoparticles composed of chitosan and iron-oxide are synthesized at room temperature by forming sequential droplets of reagents in the microreactor followed by merging and mixing them in a step by step process. The obtained nanoparticles were characterized by transmission electron microscopy, Fourier transform infrared spectrometry, and energy dispersive x-ray analysis. Results were compared with nanoparticles of the same composition synthesized with batch-wise conventional techniques. As a result, the obtained nanoparticles showed better size distribution. This microfluidic device can be used for the synthesis of other types of nanoparticles that require multi-step procedures.
Open Access
Synthesis and characterizations of water dispersible hybrid nanoparticles based on SPIONs and conjugated polymers for dual imaging applications
(2015-09) Gürbüz, Sinem
This study focuses on the synthesis and characterizations of conjugated polymer coated super-paramagnetic iron oxide nanoparticles for their potential uses in vivo and in vitro imaging. Water dispersible, stable super-paramagnetic iron oxide (SPIO) hybridized conjugated polymer nanoparticles are synthesized with three different types of conjugated polymers emitting in the region of blue, green and red. SPION, which is a T2 contrast agent due to its magnetic susceptibility, is taken into consideration because of its unique uptake mechanism by the Kupffer cells in the liver, spleen or bone marrow.[1] The core iron oxide nanoparticles are coated to increase blood circulation time, reduce the agglomeration of them and improve pharmacokinetic effect.2 Conjugated polymers utilized in this work were modified with allyl pendant groups in order to obtain cross linkable moieties. Polymer chains were cross-linked via [2+2] cycloaddition of ethylene units under UV light to confer stability .Cross-linking would not only confer stability to these hybrid nanoparticles but it can also help preventing the early leakage of SPIONs from the polymer matrix in the biological media. For this purpose, three polymers used in this study, which were poly[(9,9-bis{3-dihexyl}flourenyl-2,7-diyl)-co-(9,9-bis{3-diallyl}fluorenyl-2,7-diyl)] (PB), Poly[(9,9-bis ({3-diallyl}flourenyl-2,7-diyl)-co-(benzothiodiazole)] (PG) and poly[3-{(allyloxy)ethyl} (thiophene 2,5-diyl)-co-(5,5’-{2,2’}-bithiophene)] (P2). Nanoparticles of these polymers with and without SPIONs were synthesized. Optical and morphological characterizations were investigated via DLS, SEM, TEM, UV-Vis and Fluorescence spectroscopy.