Browsing by Subject "Erythrocyte sedimentation rate"
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Item Open Access A microfluidic erythrocyte sedimentation rate analyzer using rouleaux formation kinetics(Springer Verlag, 2017-03) Isiksacan, Z.; Asghari, M.; Elbuken, C.Red blood cell aggregation is an intrinsic property of red blood cells that form reversible stacked structures, also called rouleaux, under low shear rates. Erythrocyte sedimentation rate (ESR), commonly performed in clinics, is an indirect inflammation screener and a prognostic test for diseases. We have recently developed a microfluidic system for rapid measurement of ESR from 40 µl whole blood employing the aggregation dynamics. In this work, we propose the use of an aggregation inducer, dextran polyglucose, for the preparation of multiple blood samples with differing aggregation dynamics. Using these samples, we characterized the performance of the system with three aggregation indices and under varying experimental conditions. Additionally, using the same underlying principle, we improved the system for ESR measurement using both venipuncture and fingerprick whole blood samples depending on the user needs. The results demonstrate that the system performs equally well with both samples, which validates the compatibility of the system for both laboratory and point-of-care applications where venous and capillary blood are the primary samples, respectively. The detailed characterization presented in this study legitimates the feasibility of the system for ultrafast and facile measurement of ESR in clinics and diverse off-laboratory settings.Item Open Access Microfluidic measurement of erythrocyte sedimentation rate and monitoring of erytrocyte aggregation(Chemical and Biological Microsystems Society, 2016) Isiksacan, Ziya; Elbuken, ÇağlarWe developed a novel microfluidic opto-electro-mechanical system and performed ultrafast, cheap, and low-sample volume measurement of erythrocyte sedimentation rate (ESR) from erythrocyte aggregation (EA). We also showed EA dynamics in real-time during measurements.Item Open Access A portable microfluidic system for rapid measurement of the erythrocyte sedimentation rate(Royal Society of Chemistry, 2016) Isiksacan, Z.; Erel, O.; Elbuken, C.The erythrocyte sedimentation rate (ESR) is a frequently used 30 min or 60 min clinical test for screening of several inflammatory conditions, infections, trauma, and malignant diseases, as well as non-inflammatory conditions including prostate cancer and stroke. Erythrocyte aggregation (EA) is a physiological process where erythrocytes form face-to-face linear structures, called rouleaux, at stasis or low shear rates. In this work, we proposed a method for ESR measurement from EA. We developed a microfluidic opto-electro-mechanical system, using which we experimentally showed a significant correlation (R2 = 0.86) between ESR and EA. The microfluidic system was shown to measure ESR from EA using fingerprick blood in 2 min. 40 μl of whole blood is filled in a disposable polycarbonate cartridge which is illuminated with a near infrared emitting diode. Erythrocytes were disaggregated under the effect of a mechanical shear force using a solenoid pinch valve. Following complete disaggregation, transmitted light through the cartridge was measured using a photodetector for 1.5 min. The intensity level is at its lowest at complete disaggregation and highest at complete aggregation. We calculated ESR from the transmitted signal profile. We also developed another microfluidic cartridge specifically for monitoring the EA process in real-time during ESR measurement. The presented system is suitable for ultrafast, low-cost, and low-sample volume measurement of ESR at the point-of-care.