Browsing by Subject "Droplet merging"
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Item Open Access Development of droplet based microfluidic system for agglutination assays(Bilkent University, 2015-10) Marçalı, MerveAgglutination reactions have been carried out for several applications such as assessment of bacterial infection, blood typing or detecting non-infectious diseases. To observe agglutination reactions, several approaches have been developed. Although microfluidic methods are more costly compared to the common methods in clinical practice, microfluidic systems provide a reliable and more controlled environment. Due to the ability of droplet based systems to manipulate minute volumes of fluids, microfluidic systems allow screening of several agglutination reactions at a single run. In comparison to standard microfluidic devices, droplet based microfluidic systems provide efficient mixing which is a crucial parameter for agglutination reactions. This study reports detection of agglutination reactions of whole blood in microdroplets using impedimetric measurement method. As a proof of concept demonstration of agglutination reaction, blood typing method was implemented in microdroplets. Using label free impedimetric measurement approach, agglutination reaction in microdroplets was monitored. Impedance monitoring was achieved using microelectrodes in the microchannel to measure the impedance signal generated by droplets in the continuous phase. Besides monitoring the reaction, an empirical approach was applied to find the lumped element model of the system. This model can be used as a guidance to design a detection system and to test the sensitivity of the detection system that is used for other type of immuno-diagnostic assays in microdroplets.Item Open Access Multi-step microfludic reactor for the synthesis of hybrid nanoparticles(Institute of Physics Publishing, 2020) Wahab, Malik Abdul; Erdem, E. YeganWe introduce a multi-step microfluidic reactor for the synthesis of hybrid nanoparticles. As part of this study, nanoparticles composed of chitosan and iron-oxide are synthesized at room temperature by forming sequential droplets of reagents in the microreactor followed by merging and mixing them in a step by step process. The obtained nanoparticles were characterized by transmission electron microscopy, Fourier transform infrared spectrometry, and energy dispersive x-ray analysis. Results were compared with nanoparticles of the same composition synthesized with batch-wise conventional techniques. As a result, the obtained nanoparticles showed better size distribution. This microfluidic device can be used for the synthesis of other types of nanoparticles that require multi-step procedures.