Browsing by Subject "Doublets"

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    AMULET: a novel read count-based method for effective multiplet detection from single nucleus ATAC-seq data
    (BioMed Central Ltd., 2021-12) Thibodeau, Asa; Eroglu, Alper; McGinnis, Christopher S.; Lawlor, Nathan; Nehar-Belaid, Djamel; Kursawe, Romy; Marches, Radu; Conrad, Daniel N.; Kuchel, George A.; Gartner, Zev J.; Banchereau, Jacques; Stitzel, Michael L.; Çiçek, A. Ercüment; Ucar, Duygu
    Detecting multiplets in single nucleus (sn)ATAC-seq data is challenging due to data sparsity and limited dynamic range. AMULET (ATAC-seq MULtiplet Estimation Tool) enumerates regions with greater than two uniquely aligned reads across the genome to effectively detect multiplets. We evaluate the method by generating snATAC-seq data in the human blood and pancreatic islet samples. AMULET has high precision, estimated via donor-based multiplexing, and high recall, estimated via simulated multiplets, compared to alternatives and identifies multiplets most effectively when a certain read depth of 25K median valid reads per nucleus is achieved.