Browsing by Subject "Digital video microscopy"

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    Intracavity optical trapping with ytterbium doped fiber
    (SPIE, 2013) Laser, R.; Sayed, R.; Kalantarifard, Fatemeh; Elahi P.; İlday, F. Ömer; Volpe, Giovanni; Marago O.M.
    We propose a novel approach for trapping micron-sized particles and living cells based on optical feedback. This approach can be implemented at low numerical aperture (NA=0.5, 20X) and long working distance. In this configuration, an optical tweezers is constructed inside a ring cavity fiber laser and the optical feedback in the ring cavity is controlled by the light scattered from a trapped particle. In particular, once the particle is trapped, the laser operation, optical feedback and intracavity power are affected by the particle motion. We demonstrate that using this configuration is possible to stably hold micron-sized particles and single living cells in the focal spot of the laser beam. The calibration of the optical forces is achieved by tracking the Brownian motion of a trapped particle or cell and analysing its position distribution. © 2013 SPIE.
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    Spatial measurement of spurious forces with optical tweezers
    (SPIE, 2013) Bordeu, I.; Volpe, Giovanni; Staforelli, J. P.
    The study of diffusion in a crowded and complex environment, such as inside a cell or within a porous medium, is of fundamental importance for science and technology. Combining blinking holographic optical tweezers and sub-pixel video microscopy permits one to study Brownian motion in confined geometries. In this work, in particular, we have studied the Brownian motion of two colloidal particles interacting hydrodynamically with each other. The proximity between the two microspheres induces a space-dependence in the particles diffusion coefficient and, therefore, a spurious drift. We measure this drift and evaluate the magnitude of the spurious force associated with it. We present the optoelectronic tools employed in the experiment and we discuss the experimental results.