Browsing by Subject "Cellular imaging"

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    Cucurbit[7]uril-capped hybrid conjugated oligomer-gold nanoparticles for combined photodynamic-photothermal therapy and cellular imaging
    (ACS, 2020) Özkan, Melis; Tunç, İ.; Midilli, Y.; Ortaç, Bülend; Tuncel, Dönüş
    Herein, hybrid nanoparticles composed of a redemitting conjugated oligomer (COL) and gold nanoparticles (Au-NPs) were prepared through a one-pot synthetic method in which the oligomer acts as a reducing agent as well as a matrix to wrap the newly formed Au nanoparticles. These hybrid nanoparticles(COL-Au-NPs) exhibited photodynamic and photothermal activity against both Gram-positive and Gram-negative bacterial strains. They were also proven to possess high photostability and thermal reversibility. Dark cytotoxicity of COL-Au-NPs toward pathogens and mammalian breast cancer cells (MCF-7) reduced significantly upon complexation with cucurbit[7]uril while preserving their light-induced cytotoxic activity when irradiated with a 915 nm laser for photothermal therapy and white light for photodynamic therapy, respectively. Furthermore, these nanoparticles have cellular imaging capability because of their intrinsic fluorescence characteristics and can be used in image-guided therapy.
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    pH-responsive near-infrared emitting conjugated polymer nanoparticles for cellular imaging and controlled-drug delivery
    (John Wiley and Sons Inc., 2014) Pennakalathil, J.; Özgün, A.; Durmaz, I.; Cetin Atalay, R.; Tuncel, D.
    In this article, pH-responsive near-infrared emitting conjugated polymer nanoparticles (CPNs) are prepared, characterized, and their stabilities are investigated under various conditions. These nanoparticles have capacity to be loaded with water insoluble, anticancer drug, camptothecin (CPT), with around 10% drug loading efficiency. The in vitro release studies demonstrate that the release of CPTs from CPNs is pHdependent such that significantly faster drug release at mildly acidic pH of 5.0 compared with physiological pH 7.4 is observed. Time and dose-dependent in vitro cytotoxicity tests of blank and CPT-loaded nanoparticles are performed by realtime cell electronic sensing (RT-CES) assay with hepatocellular carcinoma cells (Huh7). The results indicate that CPNs can be effectively utilized as vehicles for pH-triggered release of anticancer drugs.