Browsing by Subject "Cell Nucleus"
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Item Open Access NAPO as a novel marker for apoptosis(The Rockefeller University Press, 2001) Sayan, B. S.; Ince, G.; Sayan, A. E.; Ozturk, M.Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody-defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.Item Open Access Polymorphism of spermatocytic seminoma. A morphometric study(Hindawi Publishing Corporation, 1994) Frasik, W.; Okoń, K.; Sokołowski, A.Spermatocytic seminoma (SS) is one of the testicular tumours. It is distinguished from other germ cell neoplasms by a special type of polymorphism. The aim of the present study was a morphometric analysis (Microplane II image system) of this phenomenon. In histological slides we measured the profile area of nuclei in about 500 adjacent cells. The basic material consisted of 12 cases (c.) of infiltrating SS, and the comparative material included intracanalicular SS (IcSS-1c.), other types of seminoma - typical (TS-2 c.), anaplastic (AS-2c.) and spermatogenesis in an elderly man (Sg-1 c.). We carried out a statistical analysis of the populations of cellular nuclei: we determined nuclear population similarities and differences, as well as their relation to various types of standard distributions such as Erlang, gamma, lognormal, normal and Weibull. Part of the SS histograms including IcSS showed similarities. The mean value of the profile area range from 32.5 to 56.5 μm2. In other types of seminomas this value was much higher. The Kolmogorov-Smirnov two-sample test showed differences between the compared nuclear populations. Lack of the differences was found only between certain SS (including IcSS). The results of comparisons of the populations studied with standard distributions showed that the analysed nuclear populations can be successfully described by more than one theoretical distribution. However, among the spermatocytic seminomas analysed, in contrast to TS and AS, we found distributions not corresponding to any of the standard distributions. The results of our findings argue also against the occurrence of haploid nuclei in SS cells. In slides from SS there were forms much larger than the nuclei of spermatocytes in Sg. In combination with the data concerning ploidy of the neoplasm we postulate that SS nuclear polymorphism is dependent both on maturation and atypia.