Browsing by Subject "Calorimetry"

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    Non-intercalative, deoxyribose binding of boric acid to calf thymus DNA
    (Springer, 2014) Ozdemir, A.; Gursacli, R. T.; Tekinay, T.
    The present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV-Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid-DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (K a) of 9.54x104 M -1. FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations. © 2014 Springer Science+Business Media.
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    Polymer/clay nanocomposites through multiple hydrogen-bonding interactions
    (John Wiley and Sons Inc., 2015) Aydin, M.; Uyar, Tamer; Tasdelen, M. A.; Yagci Y.
    An 2-ureido-4[1H]pyrimidinone (UPy) motif with self-association capability (through quadruple hydrogen bonds) was successfully anchored onto montmorillonite clay layers. Polymer/clay nanocomposites were prepared by specific hydrogen bonding interactions between surface functionalized silica nanoclays and UPy-bonded supramolecular poly(ethylene glycol) or poly(É-caprolactone). The mixed morphologies including intercalated layers with a non-uniform separation and exfoliated single layers isolated from any stack were determined by combined X-ray diffraction and transmission electron microscopic measurements. Thermal analyses showed that all nanocomposites had higher decomposition temperatures and thermal stabilities compared with neat polymer. The differential scanning calorimetric data implied that the crystallinity of polymers did not show essential changes upon introduction of organomodified UPy clays.