Browsing by Author "Rubinstein, A."
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Item Open Access Detection of a long non-coding RNA (CCAT1) in living cells and human adenocarcinoma of colon tissues using FIT–PNA molecular beacons(Elsevier Ireland Ltd., 2014-09-28) Kam, Y.; Rubinstein, A.; Naik, S.; Djavsarov, I.; Halle, D.; Ariel, I.; Gure, A. O.; Stojadinovic, A.; Pan, H. G.; Tsivin, V.; Nissan, A.; Yavin, E.Although the function and mechanism of action of long non-coding RNAs (lncRNA) is still not completely known, studies have shown their potential role in the control of gene expression and regulation, in cellular proliferation and invasiveness at the transcriptional level via multiple mechanisms. Recently, colon cancer associated transcript 1 (CCAT1) lncRNA was found to be expressed in colorectal cancer (CRC) tumors but not in normal tissue. This study aimed to study the ability of a CCAT1-specific peptide nucleic acid (PNA) based molecular beacons (TO-PNA-MB) to serve as a diagnostic probe for in vitro, ex vivo, and in situ (human colon biopsies) detection of CRC. The data showed enhanced fluorescence upon in vitro hybridization to RNA extracted from CCAT1 expressing cells (HT-29, SW-480) compared to control cells (SK-Mel-2). Uptake of TO-PNA-MBs into cells was achieved by covalently attaching cell penetrating peptides (CPPs) to the TO-PNA-MB probes. In situ hybridization of selected TO-PNA-MB in human CRC specimens was shown to detect CCAT1 expression in all (4/4) subjects with pre-cancerous adenomas, and in all (8/8) patients with invasive adenocarcinoma (penetrating the bowel wall) tumors. The results showed that CCAT1 TO-PNA-MB is a powerful diagnostic tool for the specific identification of CRC, suggesting that with the aid of an appropriate pharmaceutical vehicle, real time in vivo imaging is feasible. TO-PNA-MB may enable identifying occult metastatic disease during surgery, or differentiating in real time in vivo imaging, between benign and malignant lesions.Item Open Access An étude in modeling the definability of equilibrium(Springer Nature, 2021-10-03) Rubinstein, A.; Yıldız, KemalWe analyse the object assignment model enriched with a set of orderings over the set of agents. These orderings provide potential criteria for determining the suitability of agents to be assigned to an object. A candidate for a definable equilibrium is an assignment of the agents to the objects and an attachment of a single criterion to each object. In equilibrium, each agent is better-suited to his assigned object than any agent who envies him, according to the criterion attached to that object. We analyze the equilibrium notion and provide some examples.