Browsing by Author "Ozkan, C. S."

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    Synthesis and characterization of iron oxide derivatized mutant cowpea mosaic virus hybrid nanoparticles
    (Wiley - VCH Verlag GmbH & Co. KGaA, 2008) Martinez-Morales, A. A.; Portney, N. G.; Zhang, Y.; Destito, G.; Budak, G.; Özbay, Ekmel; Manchester, M.; Ozkan, C. S.; Ozkan, M.
    The enhanced local magnetic field strength was qualitatively analyzed by magnetic force microscopy (MFM), demonstrating a characteristic advantage for attaching derivatized magnetic iron oxide (IO) nanoparticles in an organic medium. the synthesis of 11 nm size IO nanoparticles was carried out under nitrogen atmosphere using standard schlenk technique. The biocompatible γ-Fe2O3-COOH nanoparticles were synthesized by thermal decomposition of Fe(CO)5 and surface modified. Atomic force microscopy (AFM) was used to characterize structurally the as-synthesized IO nanoparticles on a silicon substrate. The histogram of the size distribution of the IO nanoparticles determined from 68 individual measurements on single IO nanoparticles exhibited a mean size of δ11 nm. MFM showed that the textured regions observed on each hybrid are indicative of IO nanoclusters decorating the surface of single virions.
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    Zeta potential: A surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells
    (Springer New York LLC, 2008) Zhang, Y.; Yang, M.; Portney, N. G.; Cui, D.; Budak, G.; Özbay, Ekmel; Ozkan, M.; Ozkan, C. S.
    We demonstrate the use of surface Zeta potential measurements as a new tool to investigate the interactions of iron oxide nanoparticles and cowpea mosaic virus (CPMV) nanoparticles with human normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) respectively. A substantial understanding in the interaction of nanoparticles with normal and cancer cells in vitro will enable the capabilities of improving diagnostic and treatment methods in cancer research, such as imaging and targeted drug delivery. A theoretical Zeta potential model is first established to show the effects of binding process and internalization process during the nanoparticle uptake by cells and the possible trends of Zeta potential change is predicted for different cell endocytosis capacities. The corresponding changes of total surface charge of cells in the form of Zeta potential measurements were then reported after incubated respectively with iron oxide nanoparticles and CPMV nanoparticles. As observed, after MCF7 and MCF10A cells were incubated respectively with two types of nanoparticles, the significant differences in their surface charge change indicate the potential role of Zeta potential as a valuable biological signature in studying the cellular interaction of nanoparticles, as well as specific cell functionality.