Browsing by Author "Kavruk, Murat"

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Open Access
Aptamer decorated PDA@magnetic silica microparticles for bacteria purification
(SPRINGER Wien, 2024-05) Kavruk, Murat; Babaie, Zahra; Kibar, Güneş; Çetin, Barbaros; Yeşilkaya, Hasan; Amrani, Yassine; Dursun, Ali Doğan; Özalp, Veli Cengiz
One significant constraint in the advancement of biosensors is the signal-to-noise ratio, which is adversely affected by the presence of interfering factors such as blood in the sample matrix. In the present investigation, a specific aptamer binding was chosen for its affinity, while exhibiting no binding affinity towards non-target bacterial cells. This selective binding property was leveraged to facilitate the production of magnetic microparticles decorated with aptamers. A novel assay was developed to effectively isolate S. pneumoniae from PBS or directly from blood samples using an aptamer with an affinity constant of 72.8 nM. The capture experiments demonstrated efficiencies up to 87% and 66% are achievable for isolating spiked S. pneumoniae in 1 mL PBS and blood samples, respectively.
Open Access
Real-time biosensing bacteria and virus with quartz crystal microbalance: recent advances, opportunities, and challenges
(Taylor & Francis, 2023-05-16) Bonyadi, Farzaneh; Kavruk, Murat; Uçak, Samet; Çetin, Barbaros; Bayramoğlu, Gülay; Dursun, Ali D.; Arıca, Yakup; Özalp, Veli C.
Continuous monitoring of pathogens finds applications in environmental, medical, and food industry settings. Quartz crystal microbalance (QCM) is one of the promising methods for real-time detection of bacteria and viruses. QCM is a technology that utilizes piezoelectric principles to measure mass and is commonly used in detecting the mass of chemicals adhering to a surface. Due to its high sensitivity and rapid detection times, QCM biosensors have attracted considerable attention as a potential method for detecting infections early and tracking the course of diseases, making it a promising tool for global public health professionals in the fight against infectious diseases. This review first provides an overview of the QCM biosensing method, including its principle of operation, various recognition elements used in biosensor creation, and its limitations and then summarizes notable examples of QCM biosensors for pathogens, focusing on microfluidic magnetic separation techniques as a promising tool in the pretreatment of samples. The review explores the use of QCM sensors in detecting pathogens in various samples, such as food, wastewater, and biological samples. The review also discusses the use of magnetic nanoparticles for sample preparation in QCM biosensors and their integration into microfluidic devices for automated detection of pathogens and highlights the importance of accurate and sensitive detection methods for early diagnosis of infections and the need for point-of-care approaches to simplify and reduce the cost of operation.