Browsing by Author "Bayyurt, Banu"
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Item Open Access Co-encapsulating nucleic acid based TLR ligands in nanoliposomes induce potent antiviral immune activation(European Molecular Biology Organization, 2012-05) Bayyurt, Banu; Erikçi, Erdem; Gürsel, İhsanItem Open Access CpG loaded flourescent polymeric nanoparticles: a theranostic drug delivery system suitable for TLR based therapies(Turkish Society of Immunology, 2012-04) Kahraman, Tamer; Bayyurt, Banu; İbrahimova, Vusela; Tuncel, Dönüş; Gürsel, İhsanDesigning nanoparticulate delivery systems suitable for simultaneous imaging have been attracting great interest. Several systems such as liposomes, micelles, dendrimers, nanospheres and nanocapsules are potential theranostic carriers in biomedical applications. Here, for the first time we describe a complexation strategy allowing us to deliver a TLR ligand along with simultaneous imaging of the tissues with fluorescent polymeric nanoparticles (NPs). METHOD: Four different NPs (P1, P2, P3 and P4) were used for biocompatibility and drug delivery experiments. RAW264.7 cells were incubated with NPs for indicated time periods and analyzed by FACS and confocal microscopy. Cytotoxicity experiments were performed with Dojindo reagent on RAW264.7 cells. In addition, cellular uptake of NPs in the presence of several scavenger receptor ligands was also investigated. Next, NPs were incubated with CpG ODN to yield nanocomplexes. In vitro and in vivo immunostimulatory effect of nanocomplexes was analyzed both on PBMCs or splenocytes. Pro-inflammatory cytokine production was assessed either by ELISA or in some cases by PCR.Item Open Access Nanoliposomes encapsulating nucleic acid based TLR ligands induce inflammasome mediated potent immune activation(Turkish Society of Immunology, 2012-04) Bayyurt, Banu; Gürsel, İhsanItem Open Access Potent stimulation of the innate immune system by nucleic acid based TLR ligands encapsulated in nanoliposomes(Association for Cancer Immunotherapy, 2012-05) Bayyurt, Banu; Gürsel, İhsanNucleic acids with bacterial and viral origins are recognized by toll like receptors (TLRs) that triggers mammalian innate immune system to secrete proinflammatory or inflammatory cytokines. Even though these ligands have a high potency to be used in clinical applications as a vaccine adjuvant or as an immunotherapeutic agent, the rapid clearance from the body by serum proteins, in vivo degradation via nucleases, consequently lead to poor in vivo stability and performance thus hampers their clinical applications.