Browsing by Author "Aygun, Elif"

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    Antibacterial properties and osteoblast interactions of microfluidically synthesized chitosan – SPION composite nanoparticles
    (Wiley Periodicals LLC, 2023-05-26) Kafali, M.; Şahinoğlu, O. Berkay; Tufan, Y.; Orsel, Z. C.; Aygun, Elif; Alyuz, Beril; Saritas, Emine Ulku; Erdem, E. Yegan; Ercan, B.
    In this research, a multi-step microfluidic reactor was used to fabricate chitosan – superparamagnetic iron oxide composite nanoparticles (Ch – SPIONs), where composite formation using chitosan was aimed to provide antibacterial property and nanoparticle stability for magnetic resonance imaging (MRI). Monodispersed Ch – SPIONs had an average particle size of 8.8 ± 1.2 nm with a magnetization value of 32.0 emu/g. Ch – SPIONs could be used as an MRI contrast agent by shortening T2 relaxation parameter of the surrounding environment, as measured on a 3 T MRI scanner. In addition, Ch – SPIONs with concentrations less than 1 g/L promoted bone cell (osteoblast) viability up to 7 days of culture in vitro in the presence of 0.4 T external static magnetic field. These nanoparticles were also tested against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa), which are dangerous pathogens that cause infection in tissues and biomedical devices. Upon interaction of Ch – SPIONs with S. aureus and P. aeruginosa at 0.01 g/L concentration, nearly a 2-fold reduction in the number of colonies was observed for both bacteria strains at 48 h of culture. Results cumulatively showed that Ch – SPIONs were potential candidates as a cytocompatible and antibacterial agent that can be targeted to biofilm and imaged using an MRI.
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    High-throughput large scale microfluidic assembly of iron oxide nanoflowers@PS-b-PAA polymeric micelles as multimodal nanoplatforms for photothermia and magnetic imaging
    (Royal Society of Chemistry, 2023-11-17) Benassai, E.; Hortelao, A. C.; Aygun, Elif; Alpman, Aslı; Wilhelm, C.; Sarıtaş, Emine Ülkü; Abou-Hassan, A.
    Magnetic nanoparticles have been extensively explored as theranostic agents both in academic and clinical settings. Their self-assembly into nanohybrids using block copolymers can lead to new nanostructures with high functionalities and performances. Herein, we demonstrate a high-throughput and scalable method to elaborate magnetic micelles by the assembly of iron oxide magnetite nanoflowers, an efficient nanoheater, and the block copolymer Poly(styrene)-block-poly(acrylic acid) via a microfluidic-assisted nanoprecipitation method. We show that the size and shape of the magnetomicelles can be easily tuned by modulating the residence time in the microfluidic channel. In addition to their biocompatibility, we demonstrate the potential of these magnetic nanohybrids as multimodal theranostic platforms capable of generating heat by photothermia and functioning as negative contrast agents in magnetic resonance imaging and as imaging tracers in magnetic particle imaging. Notably, they outperform currently commercially available particles in terms of imaging functionalities. © 2024 RSC