Browsing by Author "Aslan, Yusuf"

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1980’lerden itibaren İslami müzik ve İslami müziğin modernleşmesi: Grup Yeniçağ
(Bilkent University, 2023) Arısan, Arda; Söylemezo, Berkay; Şevik, Dilara; Yıldız, Yiğit; Aslan, Yusuf
Bu makale, Türkiye’de 1980’lerden itibaren İslami müziğin modernleşme sürecini incelemektedir. İslami hareketin yükselişiyle birlikte, İslami müzik de dönüşüm geçirmiş ve farklı dönemlerden geçmiştir. Geleneksel İslami müzik formları, Batı müziğiyle birleştirilerek yeni bir tarz oluşturulmuştur. Grup Yeniçağ, bu modernleşme sürecinde öne çıkan gruplardan birisi olmuştur. Makalede, Grup Yeniçağ üyesi Ferhat Özgür Kale ile yapılan bir görüşme üzerine, Türkiye’de İslami müziğin modernleşme serüveni, dönemin özellikleri ve önemli aktörleri, modernleşmiş İslami müziğin toplumdaki yeri, akımın ve Türkiye’de geleneksel İslami müziğin tarihsel süreci ele alınmaktadır.
Open Access
Aptamer-based point-of-care devices: Emerging technologies and integration of computational methods
(MDPI, 2023-05-22) Aslan, Yusuf; Atabay, Maryam; Chowdhury, Hussain Kawsar; Göktürk, Ilgım; Saylan, Y.; İnci, Fatih
Recent innovations in point-of-care (POC) diagnostic technologies have paved a critical road for the improved application of biomedicine through the deployment of accurate and affordable programs into resource-scarce settings. The utilization of antibodies as a bio-recognition element in POC devices is currently limited due to obstacles associated with cost and production, impeding its widespread adoption. One promising alternative, on the other hand, is aptamer integration, i.e., short sequences of single-stranded DNA and RNA structures. The advantageous properties of these molecules are as follows: small molecular size, amenability to chemical modification, low- or nonimmunogenic characteristics, and their reproducibility within a short generation time. The utilization of these aforementioned features is critical in developing sensitive and portable POC systems. Furthermore, the deficiencies related to past experimental efforts to improve biosensor schematics, including the design of biorecognition elements, can be tackled with the integration of computational tools. These complementary tools enable the prediction of the reliability and functionality of the molecular structure of aptamers. In this review, we have overviewed the usage of aptamers in the development of novel and portable POC devices, in addition to highlighting the insights that simulations and other computational methods can provide into the use of aptamer modeling for POC integration.
Embargo
Hybrid biosensing systems for the detection of biomolecules and disease biomarkers
(2023-08) Aslan, Yusuf
Optical metasurfaces are configurations of artificially structured surfaces designed to obtain unusual electromagnetic properties. The ability to manipulate a confined electromagnetic field enables metasurfaces to be utilized as optical point-of-care (POC) biosensors for the detection of low concentrations of biomarkers. Moreover, the integration of fluorescent molecules and plasmonic metasurfaces is utilized to enhance both plasmonic and fluorescent signals; however, the nanoscale distance and spectral overlap between the fluorescent emitter and plasmonic metasurface are crucial for the separation of the fluorescence-coupled plasmonic radiation and non-radiative induced plasmon surface entrapment. In this study, fluorescently labeled (FITC) proteins are integrated over a plasmonic metasurface via three different surface modifications for obtaining a hybrid biosensing system that boosts the device’s plasmonic sensitivity and lowers the detection limit. The metasurface is fabricated via physical vapor deposition of titanium (10 nm), silver (30 nm), and gold (15 nm), respectively over polycarbonate nanograting substrates of optical disks (DVDs). Additionally, the surface modifications are arranged via short-distance, medium-distance, and long-distance modifications for fluorescently labeled molecule binding. After the evaluations, the highest plasmonic wavelength shift over the FITC labeled protein binding is obtained from the medium-distance modification with ~4.4 times signal enhancement over the short-distance modification. The medium-distance modification is further combined with an immunoassay for the detection of Alzheimer’s disease. Consequently, this study paves the way for designing new arrangements on a metasurface to couple with fluorescence molecules while enhancing the analytical performance of the plasmonic biosensor.
Embargo
Micrometasense: coupling plasmonic metasurfaces with fluorescence for enhanced detection of microplastics in real samples
(American Chemical Society, 2024-12-27) Ece, Emre; Aslan, Yusuf; Hacıosmanoglu, Nedim; İnci, Fatih
Diverse analytical techniques are employed to scrutinize microplastics (MPs)-pervasive at hazardous concentrations across diverse sources ranging from water reservoirs to consumable substances. The limitations inherent in existing methods, such as their diminished detection capacities, render them inadequate for analyzing MPs of diminutive dimensions (microplastics: 1-5 mu m; nanoplastics: < 1 mu m). Consequently, there is an imperative need to devise methodologies that afford improved sensitivity and lower detection limits for analyzing these pollutants. In this study, we introduce a holistic strategy, i.e., MicroMetaSense, reliant on a metal-enhanced fluorescence (MEF) phenomenon in detecting a myriad size and types of MPs (i.e., poly(methyl methacrylate) (PMMA) and poly(ethylene terephthalate) (PET)) down to 183-205 fg, as well as validated the system with real samples (tap and lake) and artificial ocean samples as a real-world scenario. To obtain precise size distribution in nanometer scale, MPs are initially processed with an ultrafiltration on-a-chip method, and subsequently, the MPs stained with Nile Red dye are subjected to meticulous analysis under a fluorescence microscope, utilizing both a conventional method (glass substrate) and the MicroMetaSense platform. Our approach employs a metasurface to augment fluorescence signals, leveraging the MEF phenomenon, and it demonstrates an enhancement rate of 36.56-fold in detecting MPs compared to the standardized protocols. This low-cost ($2), time-saving (under 30 min), and highly sensitive (183-205 femtogram) strategy presents a promising method for precise size distribution and notable improvements in detection efficacy not only for laboratory samples but also in real environmental samples; hence, signifying a pivotal advancement in conventional methodologies in MP detection.
Embargo
Shedding light on cellular secrets: a review of advanced optical biosensing techniques for detecting extracellular vesicles with a special focus on cancer diagnosis
(ACS Publications, 2024-08-23) Küçük, Beyza Nur; Yılmaz, Eylül Gülşen; Aslan, Yusuf; Erdem, Özgecan; İnci, Fatih
In the relentless pursuit of innovative diagnostic tools for cancer, this review illuminates the cutting-edge realm of extracellular vesicles (EVs) and their biomolecular cargo detection through advanced optical biosensing techniques with a primary emphasis on their significance in cancer diagnosis. From the sophisticated domain of nanomaterials to the precision of surface plasmon resonance, we herein examine the diverse universe of optical biosensors, emphasizing their specified applications in cancer diagnosis. Exploring and understanding the details of EVs, we present innovative applications of enhancing and blending signals, going beyond the limits to sharpen our ability to sense and distinguish with greater sensitivity and specificity. Our special focus on cancer diagnosis underscores the transformative potential of optical biosensors in early detection and personalized medicine. This review aims to help guide researchers, clinicians, and enthusiasts into the captivating domain where light meets cellular secrets, creating innovative opportunities in cancer diagnostics.