Investigation of spontaneous differentiation of neural stem cells on synthetic scaffolds

Simple item page
buir.advisorTekinay, Ayşe Begüm
dc.contributor.authorUyan, İdil
dc.date.accessioned2017-08-28T12:34:24Z
dc.date.available2017-08-28T12:34:24Z
dc.date.copyright2017-08
dc.date.issued2017-08
dc.date.submitted2017-08-24
dc.departmentGraduate Program in Neuroscienceen_US
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Neuroscience, İhsan Doğramacı Bilkent University, 2017.en_US
dc.descriptionIncludes bibliographical references (leaves 76-92).en_US
dc.description.abstractDespite the increasing incidents of brain injuries and neurodegenerative diseases, a definitive clinical therapy for these conditions has not been found yet. Nervous system injuries result in loss of neural cells, causing loss of function in the neural circuitry. As mature neurons do not divide, it is not possible to tolerate the loss of neurons by the production of new ones. In the central nervous system, even though neural stem cells are present, their number and regenerative capacity are very low. In addition, inhibitory molecules are released at the degeneration site which hinders reconnection of the remaining cells. As the damage is due to the loss of neurons, cell therapy is considered as a promising option. Neural stem cells are capable of differentiating into the three major cell types in the central nervous system: neurons, astrocytes, and oligodendrocytes. However, due to low rate of survival of the transplanted cells, there is still a need for a cell vehicle system to promote their survival, adhesion, migration, and differentiation. On the other hand, use of biological molecules such as growth factors or extracellular matrix proteins as vehicle systems should be minimized due to the immunological risks. Nanotechnological approaches serve as a great opportunity to mimic the native environment of the cells. Peptide amphiphiles (PAs) are self-assembling molecules that provide precise control over their secondary structure and the amino acid sequence, which can mimic proteins and show hydrogel properties. In this thesis, self-assembling PA scaffolds that mimic laminin, heparan sulfate and cadherin, which are key players in nervous system regeneration, have been investigated as cell delivery vehicles. Neurospheres are great models for studying the behavior of neural stem cells within a heterogeneous 3-dimensional cell population. Migration and differentiation behavior of neurospheres were investigated on laminin (LN), heparan sulfate (GAG), and cadherin-mimetic (HAV) PA nanofiber scaffolds. The results indicated that LN and GAG mimicking PA scaffolds cooperatively enhanced the migration of neurospheres, whereas cadherin mimetic PA scaffolds were individually sufficient to promote their migration. Also, a fine neural network was observed to be established on HAV-PA. These scaffolds hold high potential to be used as cell delivery vehicles.en_US
dc.description.degreeM.S.en_US
dc.description.statementofresponsibilityby İdil Uyanen_US
dc.embargo.release2020-08-23
dc.format.extentxvii, 92 leaves : illustrations (some color), charts ; 30 cm.en_US
dc.identifier.itemidB156118
dc.identifier.urihttp://hdl.handle.net/11693/33560
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNeural regenerationen_US
dc.subjectNeurospheresen_US
dc.subjectPeptide amphiphilesen_US
dc.subjectLamininen_US
dc.subjectHeparin sulfateen_US
dc.subjectCadherinen_US
dc.subjectMigrationen_US
dc.titleInvestigation of spontaneous differentiation of neural stem cells on synthetic scaffoldsen_US
dc.title.alternativeNöral kök hücrelerin sentetik platformlar üzerindeki spontane farklılaşmalarının incelenmesien_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
idil_uyan_msc_thesis_2017.pdf
Size:
4.05 MB
Format:
Adobe Portable Document Format
Description:
Full printable version
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Graduate Program in Neuroscience - Master's degree