Enhanced angiogenic effects of RGD, GHK peptides and copper (II) compositions in synthetic cryogel ECM model

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buir.contributor.authorGarifullin, Ruslan
dc.citation.epage12en_US
dc.citation.spage1en_US
dc.citation.volumeNumber120en_US
dc.contributor.authorZoughaib, M.
dc.contributor.authorLuong, D.
dc.contributor.authorGarifullin, Ruslan
dc.contributor.authorGatina, D. Z.
dc.contributor.authorFedosimova, S. V.
dc.contributor.authorAbdullin, T. I.
dc.date.accessioned2021-03-08T08:23:59Z
dc.date.available2021-03-08T08:23:59Z
dc.date.issued2020
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractSynthetic oligopeptides are a promising alternative to natural full-length growth factors and extracellular matrix (ECM) proteins in tissue regeneration and therapeutic angiogenesis applications. In this work, angiogenic properties of dual and triple compositions containing RGD, GHK peptides and copper (II) ions (Cu2+) were for the first time studied. To reveal specific in vitro effects of these compositions in three-dimensional scaffold, adamantyl group bearing peptides, namely Ada-Ahx-GGRGD (1) and Ada-Ahx-GGGHK (2), were effectively immobilized in bioinert pHEMA macroporous cryogel via host-guest β-cyclodextrin-adamantane interaction. The cryogels were additionally functionalized with Cu2+ via the formation of GHK-Cu complex. Angiogenic responses of HUVECs grown within the cryogel ECM model were analyzed. The results demonstrate that the combination of RGD with GHK and further with Cu2+ dramatically increases cell proliferation, differentiation, and production of a series of angiogenesis related cytokines and growth factors. Furthermore, the level of glutathione, a key cellular antioxidant and redox regulator, was altered in relation to the angiogenic effects. These results are of particular interest for establishing the role of multiple peptide signals on regeneration related processes and for developing improved tissue engineering materials.en_US
dc.embargo.release2023-01-01
dc.identifier.doi10.1016/j.msec.2020.111660en_US
dc.identifier.issn0928-4931
dc.identifier.urihttp://hdl.handle.net/11693/75865
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttps://dx.doi.org/10.1016/j.msec.2020.111660en_US
dc.source.titleMaterials Science and Engineering Cen_US
dc.subjectTissue regenerationen_US
dc.subjectSynthetic cryogelsen_US
dc.subjectRGDen_US
dc.subjectGHKen_US
dc.subjectCopperen_US
dc.subjectGlutathioneen_US
dc.subjectCytokinesen_US
dc.subjectTherapeutic angiogenesisen_US
dc.titleEnhanced angiogenic effects of RGD, GHK peptides and copper (II) compositions in synthetic cryogel ECM modelen_US
dc.typeArticleen_US
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Institute of Materials Science and Nanotechnology (UNAM)