Translational control of human p53 expression in yeast mediated by 5′-UTR-ORF structural interaction

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dc.citation.epage1227en_US
dc.citation.issueNumber5en_US
dc.citation.spage1222en_US
dc.citation.volumeNumber29en_US
dc.contributor.authorMokdad-Gargouri, R.en_US
dc.contributor.authorBelhadj, K.en_US
dc.contributor.authorGargouri, A.en_US
dc.date.accessioned2016-02-08T10:35:45Z
dc.date.available2016-02-08T10:35:45Z
dc.date.issued2001en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractWe have expressed human p53 cDNA in the yeast Saccharomyces cerevisiae and shown that the level of production and the lenght of the p53 protein depends on the presence of untranslated mRNA regions (UTRs). The expression of the ORF alone leads to a p53 protein of correct size (53 kDa) that accumulates to high levels, concomitantly with the presence of a small amount of a p40 protein (40 kDa). However, when either the entire 5′-UTR and a part of the 3′- or 5′-UTR alone is used, this leads to the production of small amounts of the 40 kDa truncated form only. The p40 protein corresponds to a truncated form of p53 at the C-terminal extremity since it reacts only with a monoclonal antibody recognising the N-terminal epitope. This effect on the amount and lenght of p53 protein had no correlation at the mRNA level, suggesting that translational control probably occurs through the 5′-UTR. We propose a model of structural interaction between this UTR and a part of the ORF mRNA for the regulation of p53 expression in this heterologous context.en_US
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/11693/24887
dc.language.isoEnglishen_US
dc.source.titleNucleic Acids Researchen_US
dc.subjectcomplementary DNAen_US
dc.subjectepitopeen_US
dc.subjectmessenger RNAen_US
dc.subjectmonoclonal antibodyen_US
dc.subjectprotein p40en_US
dc.subjectprotein p53en_US
dc.subject3' untranslated regionen_US
dc.subject5' untranslated regionen_US
dc.subjectamino terminal sequenceen_US
dc.subjectarticleen_US
dc.subjectcarboxy terminal sequenceen_US
dc.subjectcontrolled studyen_US
dc.subjectcorrelation functionen_US
dc.subjectgene structureen_US
dc.subjecthumanen_US
dc.subjectnonhumanen_US
dc.subjectopen reading frameen_US
dc.subjectpriority journalen_US
dc.subjectprotein expressionen_US
dc.subjectprotein synthesisen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjecttranslation regulationen_US
dc.subjecttumor suppressor geneen_US
dc.subjectyeasten_US
dc.subject3' Untranslated Regionsen_US
dc.subject5' Untranslated Regionsen_US
dc.subjectBlotting, Northernen_US
dc.subjectBlotting, Westernen_US
dc.subjectCell Divisionen_US
dc.subjectDNA, Complementaryen_US
dc.subjectGene Expression Regulationen_US
dc.subjectHumansen_US
dc.subjectOpen Reading Framesen_US
dc.subjectPlasmidsen_US
dc.subjectProtein Biosynthesisen_US
dc.subjectRNA, Messengeren_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectSequence Deletionen_US
dc.subjectTranscription, Geneticen_US
dc.subjectTumor Suppressor Protein p53en_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleTranslational control of human p53 expression in yeast mediated by 5′-UTR-ORF structural interactionen_US
dc.typeArticleen_US
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