Surface-modified bacterial nanofibrillar PHB scaffolds for bladder tissue repair
Date
2016
Authors
Karahaliloǧlu, Z.
Demirbilek, M.
Şam, M.
Saǧlam, N.
Mizrak, A. K.
Denkbaş, E. B.
Editor(s)
Advisor
Supervisor
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Co-Supervisor
Instructor
Source Title
Artificial Cells, Nanomedicine and Biotechnology
Print ISSN
2169-1401
Electronic ISSN
2169-141X
Publisher
Taylor and Francis Ltd.
Volume
44
Issue
1
Pages
74 - 82
Language
English
Type
Journal Title
Journal ISSN
Volume Title
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Abstract
The aim of the study is in vitro investigation of the feasibility of surface-modified bacterial nanofibrous poly [(R)-3-hydroxybutyrate] (PHB) graft for bladder reconstruction. In this study, the surface of electrospun bacterial PHB was modified with PEG- or EDA via radio frequency glow discharge method. After plasma modification, contact angle of EDA-modified PHB scaffolds decreased from 110 � 1.50 to 23 � 0.5 degree. Interestingly, less calcium oxalate stone deposition was observed on modified PHB scaffolds compared to that of non-modified group. Results of this study show that surface-modified scaffolds not only inhibited calcium oxalate growth but also enhanced the uroepithelial cell viability and proliferation.
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Keywords
Bladder reconstruction , Calcium oxalate stones , Nanotexture , Poly [(R)-3-hydroxybutyrate] (PHB) , Tissue engineering , Calcium , Glow discharges , Oxalic acid , Tissue , Tissue engineering , Bladder reconstruction , Calcium oxalates , Nano-texture , Plasma modifications , Poly[-3-hydroxybutyrate] , Radio frequency glow discharge , Surface-modified , Uroepithelial cells , Scaffolds (biology) , 3 hydroxybutyric acid , Calcium , Calcium oxalate , Molecular scaffold , Poly [(r) 3 hydroxybutyrate] , Unclassified drug , Biomaterial , Ethylenediamine , Ethylenediamine derivative , Hydroxybutyric acid , Macrogol derivative , Animal cell , Article , Calcium oxalate stone , Cell proliferation , Cell viability , Chemical composition , Contact angle , Crystallization , Electrospinning , Frequency , In vitro study , Nonhuman , Surface property , Tissue repair , Animal , Bladder , Cell line , Cell survival , Chemistry , Cupriavidus necator , Drug effects , Epithelium cell , Isolation and purification , Metabolism , Mouse , Nephrolithiasis , Pathology , Polymerization , Regeneration , Tissue engineering , Animals , Biocompatible materials , Calcium oxalate , Cell line , Cell proliferation , Cell survival , Cupriavidus necator , Epithelial cells , Ethylenediamines , Hydroxybutyrates , Kidney calculi , Mice , Polyethylene glycols , Polymerization , Regeneration , Tissue engineering , Urinary bladder