Affinity of glycopeptide nanofibers to growth factors and their effects on cells

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buir.advisorAdams, Michelle Marie
dc.contributor.authorHaştar, Nurcan
dc.date.accessioned2017-09-28T09:47:44Z
dc.date.available2017-09-28T09:47:44Z
dc.date.copyright2017-09
dc.date.issued2017-09
dc.date.submitted2017-09-27
dc.departmentGraduate Program in Materials Science and Nanotechnologyen_US
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Material Science and Nanotechnology, İhsan Doğramacı Bilkent University, 2017.en_US
dc.descriptionIncludes bibliographical references (leaves 53-62).en_US
dc.description.abstractThe development of scaffolds for growth factor delivery is a promising approach for tissue regeneration applications due to their crucial roles during regeneration. Growth factor secretion and interactions with glycosaminoglycans are essential steps for the regulation of cellular behavior. Therefore, glycosaminoglycan-mimetic scaffolds provide a great opportunity to modulate the effects of growth factor actions on cell fate. In this thesis, sugar-bearing peptide amphiphile molecules were characterized and tested for VEGF, FGF-2 and NGF affinity. ELISA-based affinity analyses revealed that glycopeptide nanofibers had high affinity to NGF; however, glycopeptides alone were not enough to interact efficiently with VEGF and FGF-2. Since VEGF and NGF contain heparin-binding domains, the addition of a sulfonated peptide amphiphile increased the affinity of the nanofiber network to these growth factors. Glycopeptide-sulfonate nanofibers were also found to promote in vitro tube formation through their VEGF and FGF-2 affinity. VEGF release profiles of HUVECs indicated that increasing concentration of VEGF may provide autocrine signaling and enhance tube formation without any exogenous pro-angiogenic factor addition. In addition, when NGF-responsive PC-12 cells were cultured on glycopeptide nanofibers, they extended their neurites to an extent comparable with a widely-used positive control molecule (poly-L-lysine). These results suggest that glycosaminoglycan-mimetic glycopeptide nanofiber networks can be used as efficient growth factor presentation platforms for tissue regeneration applications to induce angiogenesis or peripheral nerve regeneration.en_US
dc.description.degreeM.S.en_US
dc.description.statementofresponsibilityby Nurcan Haştar.en_US
dc.embargo.release2019-09-26
dc.format.extentxii, 62 leaves : illustrations (some color), charts ; 30 cm.en_US
dc.identifier.itemidB156503
dc.identifier.urihttp://hdl.handle.net/11693/33768
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGrowth factorsen_US
dc.subjectGlycosaminoglycansen_US
dc.subjectExtracellular matrixen_US
dc.subjectPeptide nanofibersen_US
dc.subjectNeovascularizationen_US
dc.subjectNeurite extensionen_US
dc.titleAffinity of glycopeptide nanofibers to growth factors and their effects on cellsen_US
dc.title.alternativeBüyüme faktörlerinin şekerli nanofiberlere afinitesi ve bu nanofiberlerin hücrelere etkilerien_US
dc.typeThesisen_US
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