Alternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosa

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buir.contributor.authorBilican, İsmail
buir.contributor.authorGüler, Mustafa Tahsin
dc.citation.epage11en_US
dc.citation.spage1en_US
dc.citation.volumeNumber219en_US
dc.contributor.authorBilican, İsmail
dc.contributor.authorBahadır, T.
dc.contributor.authorBilgin, K.
dc.contributor.authorGüler, Mustafa Tahsin
dc.date.accessioned2021-03-05T07:10:57Z
dc.date.available2021-03-05T07:10:57Z
dc.date.issued2020
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractPseudomonas aeruginosa is a pathogenic bacterium in fresh water supplies that creates a risk for public health. Microbiological analysis of drinking water samples is time consuming and requires qualified personnel. Here we offer a screening system for rapid analysis of spring water that has the potential to be turned into a point-of-need system by means of simple mechanism. The test, which takes 1 h to complete, electrically interrogates the particles through a microfluidic chip suspended in the water sample. We tested the platform using water samples with micro beads and water samples spiked with P. aeruginosa at various concentrations. The mono disperse micro beads were used to evaluate the performance of the system. The results were verified by the gold standard membrane filtration method, which yielded a positive test result only for the P. aeruginosa spiked samples. Detection of 0–11 k bacteria in 30 μL samples was successfully completed in 1 h and compared with a conventional microbiological method. The presented method is a good candidate for a rapid, on-site, screening test that can result in a significant reduction in cost and analysis time compared to microbiological analyses routinely used in practice.en_US
dc.embargo.release2022-11-01
dc.identifier.doi10.1016/j.talanta.2020.121293en_US
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/11693/75814
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttps://dx.doi.org/10.1016/j.talanta.2020.121293en_US
dc.source.titleTalantaen_US
dc.subjectMicrofluidic chipen_US
dc.subjectElectrical detectionen_US
dc.subjectP. aeruginosaen_US
dc.subjectBacteriaen_US
dc.subjectWater analysisen_US
dc.titleAlternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosaen_US
dc.typeArticleen_US
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Institute of Materials Science and Nanotechnology (UNAM)
Nanotechnology Research Center (NANOTAM)