The Turkish bath structure (hamam) is one of the key typologies in Anatolian architecture. In addition to its main “bathing” function, Turkish baths are chosen as the main venue for social organizations in the Ottoman period, with eating and dancing accompanied by live music. Thus, the construction of baths, in various sizes, was prioritized then and still holds its place in the social and cultural life of Anatolia. This study investigates the authentic acoustical characteristics of Turkish baths, over four selected baths in Bursa, Turkey; Karamustafa (15th c.), Yeni Kaplıca (16th c.), Kaynarca (17th c.), and Tahirağa (19th c.). Room impulse responses are collected through acoustical field measurements. Acoustical simulations are utilized to experiment with the materials, which reflect the historical origins, as well as used to test the effects of different humidity levels on acoustical parameters. The objective parameter analysis includes EDT, T20, T30, C80, D50, and STI assessments. Relationships between objective parameters and geometrical attributes are investigated. Lower T30 and higher STI values are obtained with historical plaster in comparison to the up-to-date conditions. Controlled analysis in decreasing the relative humidity resulted in lower T20 and T30 values, consequently higher STI values. Obtained data are discussed considering the usage of male and female baths, traditional activities as well as the nature of the sound sources.