Long-term acetylcholinesterase depletion alters the levels of key synaptic proteins while maintaining neuronal markers in the aging zebrafish (Danio rerio) Brain

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buir.contributor.authorKaroğlu-Eravsar, Elif Tuğçe
buir.contributor.authorTüz-Şaşik, Melek Umay
buir.contributor.authorKaraduman, Ayşenur
buir.contributor.authorKeşküş, Ayse Gökçe
buir.contributor.authorArslan-Ergul, Ayça
buir.contributor.authorKonu, Özlen
buir.contributor.authorKafalıgönül, Hulusi
buir.contributor.authorAdams, Michelle M.
buir.contributor.orcidKaroğlu-Eravsar, Elif Tuğçe|0000-0001-5110-3956
buir.contributor.orcidTüz-Şaşik, Melek Umay|0000-0002-0906-7477
buir.contributor.orcidKaraduman, Ayşenur|0000-0002-8002-6571
buir.contributor.orcidKeşküş, Ayse Gökçe|0000-0002-3934-8587
buir.contributor.orcidArslan-Ergul, Ayça|0000-0001-7024-907X
buir.contributor.orcidKonu, Özlen|0000-0002-6223-5329
buir.contributor.orcidKafalıgönül, Hulusi|0000-0001-5033-4138
dc.citation.epage1436en_US
dc.citation.issueNumber12
dc.citation.spage1424
dc.citation.volumeNumber69
dc.contributor.authorKaroğlu-Eravsar, Elif Tuğçe
dc.contributor.authorTüz-Şaşik, Melek Umay
dc.contributor.authorKaraduman, Ayşenur
dc.contributor.authorKeşküş, Ayse Gökçe
dc.contributor.authorArslan-Ergul, Ayça
dc.contributor.authorKonu, Özlen
dc.contributor.authorKafalıgönül, Hulusi
dc.contributor.authorAdams, Michelle M.
dc.date.accessioned2024-03-14T11:20:47Z
dc.date.available2024-03-14T11:20:47Z
dc.date.issued2023-10-04
dc.departmentDepartment of Molecular Biology and Genetics
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)
dc.departmentNational Magnetic Resonance Research Center (UMRAM)
dc.description.abstractIntroduction: Interventions targeting cholinergic neurotransmission like acetylcholinesterase (AChE) inhibition distinguish potential mechanisms to delay age-related impairments and attenuate deficits related to neurodegenerative diseases. However, the chronic effects of these interventions are not well described. Methods: In the current study, global levels of cholinergic, cellular, synaptic, and inflammation-mediating proteins were assessed within the context of aging and chronic reduction of AChE activity. Long-term depletion of AChE activity was induced by using a mutant zebrafish line, and they were compared with the wildtype group at young and old ages. Results: Results demonstrated that AChE activity was lower in both young and old mutants, and this decrease coincided with a reduction in ACh content. Additionally, an overall age-related reduction in AChE activity and the AChE/ACh ratio was observed, and this decline was more prominent in wildtype groups. The levels of an immature neuronal marker were upregulated in mutants, while a glial marker showed an overall reduction. Mutants had preserved levels of inhibitory and presynaptic elements with aging, whereas glutamate receptor subunit levels declined. Conclusion: Long-term AChE activity depletion induces synaptic and cellular alterations. These data provide further insights into molecular targets and adaptive responses following the long-term reduction of AChE activity that was also targeted pharmacologically to treat neurodegenerative diseases in human subjects.
dc.identifier.doi10.1159/000534343
dc.identifier.eissn1423-0003
dc.identifier.issn0304-324X
dc.identifier.urihttps://hdl.handle.net/11693/114749
dc.language.isoEnglish
dc.publisherS. Karger AG
dc.relation.isversionofhttps://dx.doi.org/10.1159/000534343
dc.source.titleGerontology
dc.subjectAcetylcholinesterase
dc.subjectAging
dc.subjectCholinergic system
dc.subjectNeuronal changes
dc.subjectSynapses
dc.titleLong-term acetylcholinesterase depletion alters the levels of key synaptic proteins while maintaining neuronal markers in the aging zebrafish (Danio rerio) Brain
dc.typeArticle
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Department of Molecular Biology and Genetics
Aysel Sabuncu Brain Research Center (BAM)
Department of Psychology
Institute of Materials Science and Nanotechnology (UNAM)
National Magnetic Resonance Research Center (UMRAM)