Analysis of monodisperse microfluidic droplet generation and its biochemical applications

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buir.advisorElbüken, Çağlar
dc.contributor.authorKalantarifard, Ali
dc.date.accessioned2020-12-03T13:45:25Z
dc.date.available2020-12-03T13:45:25Z
dc.date.copyright2020-11
dc.date.issued2020-11
dc.date.submitted2020-12-02
dc.departmentGraduate Program in Materials Science and Nanotechnologyen_US
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (Ph.D.): Bilkent University, Department of Materials Science and Nanotechnology, İhsan Doğramacı Bilkent University, 2020.en_US
dc.descriptionIncludes bibliographical references (leaves 93-103)en_US
dc.description.abstractDroplet fluidic systems have dramatically improved precision in many applications, such as polymerase chain reaction, biochemical analysis, and particle synthesis in which accurate control of sample volume plays a significant role. Despite the well-understood physics of squeezing regime droplet formation in two-phase flow systems, the long-sought-after goal of generating identical, equal size droplets is challenging. Although the individual parameters that affect the droplet size were identified as channel dimension, wettability, viscosity, and flow rate or pressure ratio of the two immiscible fluids, the governing mechanism of droplet size variation is not completely analyzed. More importantly, the limit of monodispersity for droplet generation systems is still unknown. This is due to the difficulty in analytical modeling of droplet formation that is usually compensated by experimental approaches, which fall short in leading to universal conclusions. In this thesis, depending on the flow source used for driving fluids we present an analytical approach that takes into account all the system dynamics and internal and external factors that disturb monodispersity. We use the analogy between fluidic and electrical circuits to analyze the factors that influence droplet monodispersity. Interestingly, we enable to model the dynamics of a segmented two-phase flow system using a single-phase flow analogy, electron flow, in electrical circuits. Doing so, we reveal the sources of disturbances that lead to variation in droplet volume. We offered a unique solution and designed guidelines to ensure ultramonodisperse droplet generation. Our analytical conclusions are experimentally verified using a T-junction and flow-focusing droplet generator design driven by a pressure supply. Equally importantly, we show the limiting experimental factors for reaching the theoretical maximum of monodispersity. For the displacement pump case, we propose a more effective and widely applicable solution to improve flow stability, by controlling off-chip compliances to minimize fluctuations due to the flow source. Eventually, we compare the performance of the two common drive units (pressure-driven and displacement pump) in terms of droplet monodispersity, while using our proposed methods and guidelines. Finally, we did study in reaction kinetics of poly dopamine and hydrogen peroxide and synthesize silica and polyethylene glycol (PEG) particles and supramolecular polymer capsules with high monodispersity using ultra-monodisperse droplets.en_US
dc.description.degreePh.D.en_US
dc.description.statementofresponsibilityby Ali Kalantarifarden_US
dc.format.extentxvii, 106 leaves : color illustratıons, charts ; 30 cm.en_US
dc.identifier.itemidB122606
dc.identifier.urihttp://hdl.handle.net/11693/54834
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicrofluidicsen_US
dc.subjectMicrodropleten_US
dc.subjectDroplet monodispersityen_US
dc.subjectParticle synthesisen_US
dc.titleAnalysis of monodisperse microfluidic droplet generation and its biochemical applicationsen_US
dc.title.alternativeEşdağılımlı mikroakışkan damlacık oluşumunun analizi ve biyokimyasal uygulamalarıen_US
dc.typeThesisen_US
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