Biomineralization of calcium phosphate crystals controlled by protein-protein interactions

Simple item page
buir.contributor.authorDuman, Elif
buir.contributor.authorŞahin-Kehribar, Ebru
buir.contributor.authorAhan, Recep Erdem
buir.contributor.authorYuca, Esra
buir.contributor.authorŞeker, Urartu Özgür Şafak
dc.citation.epage4763en_US
dc.citation.issueNumber9en_US
dc.citation.spage4750en_US
dc.citation.volumeNumber5en_US
dc.contributor.authorDuman, Elifen_US
dc.contributor.authorŞahin-Kehribar, Ebruen_US
dc.contributor.authorAhan, Recep Erdemen_US
dc.contributor.authorYuca, Esraen_US
dc.contributor.authorŞeker, Urartu Özgür Şafaken_US
dc.date.accessioned2020-02-12T06:11:23Z
dc.date.available2020-02-12T06:11:23Z
dc.date.issued2019
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractHydroxyapatite (HAP) is the major biomineral of bone. Despite the large number of studies addressing HAP formation, a fundamental understanding of the critical roles of HAP-forming proteins in vitro is needed. Effects of two HAP-interacting proteins, osteocalcin (OCN) and osteopontin (OPN), on HAP formation was investigated via in vitro biomineralization experiments, and their outcomes on the crystal structure of calcium phosphate (CaP) was revealed. Our data suggest that OCN concentration is negatively correlated with crystal formation rate and crystal size, yet the presence of OCN leads to a more ordered HAP crystal formation. On the other hand, OPN protein promotes faster formation of CaP crystals potentially working as a growth site for mineral formation, and it decreases the Ca:P ratio. This effect results in a shift from HAP-type minerals to less ordered crystals. The crystal size, shape, and Ca:P ratio can be tuned to design improved mammalian hard tissue environment-mimicking matrices by taking advantage of the OCN and OPN proteins on crystal formation. We believe our current findings will lead to innovative approaches for bone biomineralization in regenerative medicine.en_US
dc.identifier.doi10.1021/acsbiomaterials.9b00649en_US
dc.identifier.issn2373-9878
dc.identifier.urihttp://hdl.handle.net/11693/53292
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttps://dx.doi.org/10.1021/acsbiomaterials.9b00649en_US
dc.source.titleACS Biomaterials Science and Engineeringen_US
dc.subjectBiomineralizationen_US
dc.subjectHydroxyapatite crystalsen_US
dc.subjectProtein−protein interactionen_US
dc.titleBiomineralization of calcium phosphate crystals controlled by protein-protein interactionsen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Biomineralization_of_calcium_phosphate_crystals_controlled_by_protein_protein_interactions.pdf
Size:
8.31 MB
Format:
Adobe Portable Document Format
Description:
View / Download
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Institute of Materials Science and Nanotechnology (UNAM)
Nanotechnology Research Center (NANOTAM)