Multiplex systems for the amplification of short tandem repeat loci: evaluation of laser fluorescence detection

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dc.citation.epage747en_US
dc.citation.issueNumber4en_US
dc.citation.spage742en_US
dc.citation.volumeNumber23en_US
dc.contributor.authorRicciardone, M. D.en_US
dc.contributor.authorLins, A. M.en_US
dc.contributor.authorSchumm, J. W.en_US
dc.contributor.authorHolland, M. M.en_US
dc.date.accessioned2016-02-08T10:47:27Z
dc.date.available2016-02-08T10:47:27Z
dc.date.issued1997en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractShort tandem repeat (STR) loci are ideal markers for personal identification and for genomic mapping. Two fluorescent multiplex systems, each designed for simultaneous PCR amplification of four polymorphic STR loci (HUMCSF1PO, HUMTPOX, HUMTH01 and HUMVWFA31, and HUMF13A01, HUMFESFPS, HUMBFXIII and HUMLIPOL), were evaluated on three laser fluorescence detection instruments. Concordant DNA typing results were obtained with all three detection methods. These fluorescent multiplex STR systems offer an accurate, reproducible and versatile method of DNA profiling that is well-suited for forensic identity testing and other genetic analyses.en_US
dc.identifier.issn0736-6205
dc.identifier.urihttp://hdl.handle.net/11693/25595
dc.language.isoEnglishen_US
dc.source.titleBioTechniquesen_US
dc.subjectDNA sequenceen_US
dc.subjectFluorescenceen_US
dc.subjectGel electrophoresisen_US
dc.subjectGene amplificationen_US
dc.subjectGene mappingen_US
dc.subjectGenetic analysisen_US
dc.subjectProtein denaturationen_US
dc.subjectTandem repeaten_US
dc.subjectAllelesen_US
dc.subjectDNAen_US
dc.subjectFluorometryen_US
dc.subjectLasersen_US
dc.titleMultiplex systems for the amplification of short tandem repeat loci: evaluation of laser fluorescence detectionen_US
dc.typeArticleen_US
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