Fluorescence detection of biological thiols and axially chiral bodipy derivatives and alternative methodologies for singlet oxygen generation for photodynamic action

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buir.advisorAkkaya, Engin U.
dc.contributor.authorKölemen, Safacan
dc.date.accessioned2016-01-08T20:19:50Z
dc.date.available2016-01-08T20:19:50Z
dc.date.issued2014
dc.departmentGraduate Program in Materials Science and Nanotechnologyen_US
dc.descriptionAnkara : Materials Science and Nanotechnology Program of the Graduate School of Engineering and Science of Bilkent University, 2014.en_US
dc.descriptionThesis (Ph. D.) -- Bilkent University, 2014.en_US
dc.descriptionIncludes bibliographical references leaves 173-186.en_US
dc.description.abstractCalorimetric and luminescent detection of biological thiols namely cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) have attracted great interest due to the their biological significance. There are many reported fluorescent probes for Cys and Hcy, however selective probe designs for GSH remained elusive. We represented in thesis (Chapter 3) a BODIPY based selective fluorescent probe for the in vitro detection of GSH in cancer cell lines. Photodynamic therapy (PDT) is one of the promising and developing treatment modality for certain indications. Therapeutic action is achieved by the generation of cytotoxic singlet oxygen (SO). Most critical compartment of SO production pathway is the sensitizer molecule. In order to get effective inter-system crossing, which is highly needed for singlet oxygen generation, common strategy is to incorporate heavy atoms on sensitizers. However, presence of heavy atoms increases the dark toxicity that is not desired in clinical applications. In Chapter 4, we are introducing a new concept for activatable heavy atom free sensitization of PDT by designing novel orthogonal BODIPY derivatives and detailed computational analysis of this new concept. While dealing with orthogonal BODIPYs, we synthesized for the first time two axially chiral BODIPY derivatives and characterized the enantiopure products, which holds great promise for enantioselective sensing applications (Chapter 5). PDT has two major problems, which are light penetration depth of the incident light and the hypoxia. These two restrictions are addressed in chapter 6, by combining gold nanorods and aromatic endoperoxides.en_US
dc.description.degreePh.D.en_US
dc.description.statementofresponsibilityKölemen, Safacanen_US
dc.embargo.release2016-11-12
dc.format.extentxxvi, 225 leaves, illustrations, graphicsen_US
dc.identifier.itemidB148945
dc.identifier.urihttp://hdl.handle.net/11693/18497
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGSH probeen_US
dc.subjectAxial chiralityen_US
dc.subjectPhotodynamic therapyen_US
dc.subjectSinglet oxygenen_US
dc.subjectGold nanorods/endoperoxidesen_US
dc.subject.lccQZ267 .K65 2014en_US
dc.subject.lcshPhotochemotherapy.en_US
dc.subject.lcshCancer--Photochemotherapy.en_US
dc.subject.lcshOxidation.en_US
dc.subject.lcshThiols.en_US
dc.titleFluorescence detection of biological thiols and axially chiral bodipy derivatives and alternative methodologies for singlet oxygen generation for photodynamic actionen_US
dc.typeThesisen_US
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