Zebrafish brain RNA sequencing reveals that cell adhesion molecules are critical in brain aging
Author(s)
Date
2020Source Title
Neurobiology of Aging
Print ISSN
0197-4580
Publisher
Elsevier
Volume
94
Pages
164 - 175
Language
English
Type
ArticleItem Usage Stats
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Abstract
Brain aging is a complex process, which involves multiple pathways including various components from cellular to molecular. This study aimed to investigate the gene expression changes in zebrafish brains through young-adult to adult, and adult to old age. RNA sequencing was performed on isolated neuronal cells from zebrafish brains. The cells were enriched in progenitor cell markers, which are known to diminish throughout the aging process. We found 176 statistically significant, differentially expressed genes among the groups, and identified a group of genes based on gene ontology descriptions, which were classified as cell adhesion molecules. The relevance of these genes was further tested in another set of zebrafish brains, human healthy, and Alzheimer’s disease brain samples, as well as in Allen Brain Atlas data. We observed that the expression change of 2 genes, GJC2 and ALCAM, during the aging process was consistent in all experimental sets. Our findings provide a new set of markers for healthy brain aging and suggest new targets for therapeutic approaches to neurodegenerative diseases.
Permalink
http://hdl.handle.net/11693/75823Published Version (Please cite this version)
https://dx.doi.org/10.1016/j.neurobiolaging.2020.04.017Collections
- Aysel Sabuncu Brain Research Center (BAM) 228
- Department of Computer Engineering 1510
- Department of Molecular Biology and Genetics 512
- Department of Psychology 216
- Institute of Materials Science and Nanotechnology (UNAM) 2098
- Nanotechnology Research Center (NANOTAM) 1125
- National Magnetic Resonance Research Center (UMRAM) 250