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      Dietary and pharmacological interventions that inhibit mammalian target of rapamycin activity alter the brain expression levels of neurogenic and glial markers in an age-and treatment-dependent manner

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      Author(s)
      Çelebi-Birand, Dilan
      Ardıç, Narin İlgim
      Karoğlu-Eravşar, Elif Tuğçe
      Şengül, Göksemin Fatma
      Kafalıgönül, Hulusi
      Adams, Michelle M.
      Date
      2020
      Source Title
      Rejuvenation Research
      Print ISSN
      1549-1684
      Publisher
      Mary Ann Liebert
      Volume
      23
      Issue
      6
      Pages
      485 - 497
      Language
      English
      Type
      Article
      Item Usage Stats
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      260
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      Abstract
      Intermittent fasting (IF) and its mimetic, rapamycin extend lifespan and healthspan through mechanisms that are not fully understood. We investigated different short-term durations of IF and rapamycin on cellular and molecular changes in the brains of young (6–10 months) and old (26–31 months) zebrafish. Interestingly, our results showed that IF significantly lowered glucose levels while increasing DCAMKL1 in both young and old animals. This proliferative effect of IF was supported by the upregulation of foxm1 transcript in old animals. Rapamycin did not change glucose levels in young and old animals but had differential effects depending on age. In young zebrafish, proliferating cell nuclear antigen and the LC3-II/LC3-I ratio was decreased, whereas glial fibrillary acidic protein and gephyrin were decreased in old animals. The changes in proliferative markers and a marker of autophagic flux suggest an age-dependent interplay between autophagy and cell proliferation. Additionally, changes in glia and inhibitory tone suggest a suppressive effect on neuroinflammation but may push the brain toward a more excitable state. Mammalian target of rapamycin (mTOR) activity in the brain following the IF and rapamycin treatment was differentially regulated by age. Interestingly, rapamycin inhibited mTOR more potently in young animals than IF. Principal component analysis supported our conclusion that the regulatory effects of IF and rapamycin were age-specific, since we observed different patterns in the expression levels and clustering of young and old animals. Taken together, our results suggest that even a short-term duration of IF and rapamycin have significant effects in the brain at young and old ages, and that these are age and treatment dependent.
      Keywords
      Aging
      Brain
      Zebrafish
      Intermittent fasting
      Rapamycin
      mTOR signaling
      Permalink
      http://hdl.handle.net/11693/75754
      Published Version (Please cite this version)
      https://dx.doi.org/10.1089/rej.2019.2297
      Collections
      • Aysel Sabuncu Brain Research Center (BAM) 249
      • Department of Molecular Biology and Genetics 542
      • Department of Psychology 242
      • Institute of Materials Science and Nanotechnology (UNAM) 2258
      • National Magnetic Resonance Research Center (UMRAM) 301
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