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      Biotin functionalized self‐assembled peptide nanofiber as an adjuvant for immunomodulatory response

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      Embargo Lift Date: 2021-12-07
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      Author(s)
      Demircan, Muhammed Burak
      Tohumeken, Sehmus
      Gündüz, Nuray
      Khalily, Mohammad Aref
      Tekinay, T.
      Güler, M. O.
      Tekinay, Ayşe B.
      Date
      2020-12
      Source Title
      Biotechnology Journal
      Print ISSN
      1860-6768
      Publisher
      Wiley-VCH Verlag
      Volume
      15
      Issue
      12
      Pages
      2000100
      Language
      English
      Type
      Article
      Item Usage Stats
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      146
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      Abstract
      Biotinylated peptide amphiphile (Biotin‐PA) nanofibers, are designed as a noncovalent binding location for antigens, which are adjuvants to enhance, accelerate, and prolong the immune response triggered by antigens. Presenting antigens on synthetic Biotin‐PA nanofibers generated a higher immune response than the free antigens delivered with a cytosine‐phosphate‐guanine oligodeoxynucleotides (CpG ODN) (TLR9 agonist) adjuvant. Antigen attached Biotin‐PA nanofibers trigger splenocytes to produce high levels of cytokines (IFN‐γ, IL‐12, TNF‐α, and IL‐6) and to exhibit a superior cross‐presentation of the antigen. Both Biotin‐PA nanofibers and CpG ODN induce a Th‐1‐biased IgG subclass response; however, delivering the antigen with Biotin‐PA nanofibers induce significantly greater production of total IgG and subclasses of IgG compared to delivering the antigen with CpG ODN. Contrary to CpG ODN, Biotin‐PA nanofibers also enhance antigen‐specific splenocyte proliferation and increase the proportion of the antigen‐specific CD8(+) T cells. Given their biodegradability and biocompatibility, Biotin‐PA nanofibers have a significant potential in immunoengineering applications as a biomaterial for the delivery of a diverse set of antigens derived from intracellular pathogens, emerging viral diseases such as COVID‐19, or cancer cells to induce humoral and cellular immune responses against the antigens.
      Keywords
      Adjuvant
      Peptide nanofiber
      Self‐assembly
      Vaccine
      Virus‐like particles
      Permalink
      http://hdl.handle.net/11693/75650
      Published Version (Please cite this version)
      https://dx.doi.org/10.1002/biot.202000100
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