• About
  • Policies
  • What is open access
  • Library
  • Contact
Advanced search
      View Item 
      •   BUIR Home
      • Scholarly Publications
      • Faculty of Engineering
      • Department of Electrical and Electronics Engineering
      • View Item
      •   BUIR Home
      • Scholarly Publications
      • Faculty of Engineering
      • Department of Electrical and Electronics Engineering
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      High-throughput, high-resolution interferometric light microscopy of biological nanoparticles

      Thumbnail
      View / Download
      5.1 Mb
      Author(s)
      Yurdakul, C.
      Avcı, O.
      Matlock, A.
      Devaux, A. J.
      Quintero, M. V.
      Özbay, Ekmel
      Davey, R. A.
      Connor, J. H.
      Karl, W. C.
      Tian, L.
      Ünlü, M. Selim
      Date
      2020-01
      Source Title
      ACS Nano
      Print ISSN
      1936-0851
      Publisher
      American Chemical Society
      Volume
      14
      Issue
      22
      Pages
      2002 - 2013
      Language
      English
      Type
      Article
      Item Usage Stats
      99
      views
      351
      downloads
      Abstract
      Label-free, visible light microscopy is an indispensable tool for studying biological nanoparticles (BNPs). However, conventional imaging techniques have two major challenges: (i) weak contrast due to low-refractive-index difference with the surrounding medium and exceptionally small size and (ii) limited spatial resolution. Advances in interferometric microscopy have overcome the weak contrast limitation and enabled direct detection of BNPs, yet lateral resolution remains as a challenge in studying BNP morphology. Here, we introduce a wide-field interferometric microscopy technique augmented by computational imaging to demonstrate a 2-fold lateral resolution improvement over a large field-of-view (>100 × 100 μm2 ), enabling simultaneous imaging of more than 104 BNPs at a resolution of ∼150 nm without any labels or sample preparation. We present a rigorous vectorial-optics-based forward model establishing the relationship between the intensity images captured under partially coherent asymmetric illumination and the complex permittivity distribution of nanoparticles. We demonstrate high-throughput morphological visualization of a diverse population of Ebola virus-like particles and a structurally distinct Ebola vaccine candidate. Our approach offers a low-cost and robust label-free imaging platform for high-throughput and high-resolution characterization of a broad size range of BNPs.
      Keywords
      Interference microscopy
      Nanoparticle detection
      Biosensing
      Computational imaging
      Label-free
      Fourier optics
      Permalink
      http://hdl.handle.net/11693/55118
      Published Version (Please cite this version)
      https://dx.doi.org/10.1021/acsnano.9b08512
      Collections
      • Department of Electrical and Electronics Engineering 4011
      • Department of Physics 2550
      • Institute of Materials Science and Nanotechnology (UNAM) 2258
      • Nanotechnology Research Center (NANOTAM) 1179
      Show full item record

      Browse

      All of BUIRCommunities & CollectionsTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsCoursesThis CollectionTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsCourses

      My Account

      Login

      Statistics

      View Usage StatisticsView Google Analytics Statistics

      Bilkent University

      If you have trouble accessing this page and need to request an alternate format, contact the site administrator. Phone: (312) 290 2976
      © Bilkent University - Library IT

      Contact Us | Send Feedback | Off-Campus Access | Admin | Privacy