• About
  • Policies
  • What is open access
  • Library
  • Contact
Advanced search
      View Item 
      •   BUIR Home
      • University Library
      • Bilkent Theses
      • Theses - Graduate Program in Neuroscience
      • Graduate Program in Neuroscience - Master's degree
      • View Item
      •   BUIR Home
      • University Library
      • Bilkent Theses
      • Theses - Graduate Program in Neuroscience
      • Graduate Program in Neuroscience - Master's degree
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Effects of varying levels and differing durations of calorie restriction on cellular and synaptic proteins, as well as the inflammatory state of the female mouse brain

      Thumbnail
      Embargo Lift Date: 2021-04-13
      View / Download
      3.5 Mb
      Author(s)
      Macaroğlu, Duygu
      Advisor
      Adams, Michelle Marie
      Date
      2020-10
      Publisher
      Bilkent University
      Language
      English
      Type
      Thesis
      Item Usage Stats
      83
      views
      1
      downloads
      Abstract
      Aging is an inevitable and complicated process leading to functional decline. Regarding brain aging, cognitive decline takes place in multiple domains, including learning, memory, executive functions, motor coordination, and language. At the cellular and molecular level, age-related cognitive decline is elucidated with certain hallmarks, including aberrant neuronal network, stem cell exhaustion, glial cell activation, and inflammation. Calorie restriction (CR) is a widely-utilized approach for coping with aging’s detrimental effects even though there is no one agreed way for the application of CR. In this study, varying levels of CRs were applied for differing durations to MMTV-TGF-alpha female mice. The study initiated when mice were 10-weeks of age (Baseline) and carried out until 49/50 weeks of age and 81/82 weeks of age. There were four dietary groups named Adlibitum (AL; control), Chronic Calorie Restriction (CCR), Intermittent Calorie Restriction - Restriction (ICR-R), and Intermittent Calorie Restriction -Refeed (ICR-RF). The study’s first aim was to show age-related changes in the cellular and synaptic proteins and the inflammatory state of the female mice’s brains. The second aim of the study is to demonstrate the effects of varying levels of CR implemented for the short-term and the long-term manner on the same hallmarks. Our findings showed both chronic- or intermittent- CR altered the synaptic integrity proteins against brain aging at the long-term period (81/82 weeks) compared to the short-term (49/50 weeks) period except for PSD-95. Similarly, both chronic- or intermittent- CR showed an attenuative impact on the pro-inflammatory markers, but IL-6 was affected only by CCR at the same periods. Furthermore, an age-related imbalance between neurogenesis and astrogliogenesis was shown based on DCX and GFAP. Both chronic- or intermittent- CR showed a compensatory effect on it acting through astrogliogenesis, even though it was not statistically significant.
      Keywords
      Brain aging
      Calorie restriction
      Proliferation
      Synaptic integrity
      Neuroinflammation
      Western blot
      Female mouse
      Permalink
      http://hdl.handle.net/11693/54219
      Collections
      • Graduate Program in Neuroscience - Master's degree 38
      Show full item record

      Browse

      All of BUIRCommunities & CollectionsTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsThis CollectionTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartments

      My Account

      LoginRegister

      Statistics

      View Usage StatisticsView Google Analytics Statistics

      Bilkent University

      If you have trouble accessing this page and need to request an alternate format, contact the site administrator. Phone: (312) 290 1771
      © Bilkent University - Library IT

      Contact Us | Send Feedback | Off-Campus Access | Admin | Privacy