• About
  • Policies
  • What is openaccess
  • Library
  • Contact
Advanced search
      View Item 
      •   BUIR Home
      • Scholarly Publications
      • Aysel Sabuncu Brain Research Center (BAM)
      • View Item
      •   BUIR Home
      • Scholarly Publications
      • Aysel Sabuncu Brain Research Center (BAM)
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Estrogen and aging affect synaptic distribution of phosphorylated LIM kinase (pLIMK) in CA1 region of female rat hippocampus

      Thumbnail
      View / Download
      262.7 Kb
      Author
      Yildirim, M.
      Janssen, W. G. M.
      Tabori, N. E.
      Adams, Michelle M.
      Yuen, G. S.
      Akama, K. T.
      McEwen, B. S.
      Milner, T. A.
      Morrison, J. H.
      Date
      2008
      Source Title
      Neuroscience
      Print ISSN
      0306-4522
      Publisher
      Elsevier
      Volume
      152
      Issue
      2
      Pages
      360 - 370
      Language
      English
      Type
      Article
      Item Usage Stats
      72
      views
      52
      downloads
      Abstract
      17β-Estradiol (E) increases axospinous synapse density in the hippocampal CA1 region of young female rats, but not in aged rats. This may be linked to age-related alterations in signaling pathways activated by synaptic estrogen receptor α (ER-α) that potentially regulate spine formation, such as LIM-kinase (LIMK), an actin depolymerizing factor/cofilin kinase. We hypothesized that, as with ER-α, phospho-LIM-kinase (pLIMK) may be less abundant or responsive to E in CA1 synapses of aged female rats. To address this, cellular and subcellular distribution of pLIMK-immunoreactivity (IR) in CA1 was analyzed by light and electron microscopy in young and aged female rats that were ovariectomized and treated with either vehicle or E. pLIMK-IR was found primarily in perikarya within the pyramidal cell layer and dendritic shafts and spines in stratum radiatum (SR). While pLIMK-IR was occasionally present in terminals, post-embedding quantitative analysis of SR showed that pLIMK had a predominant post-synaptic localization and was preferentially localized within the postsynaptic density (PSD). The percentage of pLIMK-labeled synapses increased (30%) with E treatment (P<0.02) in young animals, and decreased (43%) with age (P<0.002) regardless of treatment. The pattern of distribution of pLIMK-IR within dendritic spines and synapses was unaffected by age or E treatment, with the exception of an E-induced increase in the non-synaptic core of spines in young females. These data suggest that age-related synaptic alterations similar to those seen with ER-α occur with signaling molecules such as pLIMK, and support the hypothesis that age-related failure of E treatment to increase synapse number in CA1 may be due to changes in the molecular profile of axospinous synapses with respect to signaling pathways linked to formation of additional spines and synapses in response to E.
      Keywords
      Sex steroids
      Electron microscopy
      Immunogold
      Signal transduction
      Plasticity
      Synapse
      Permalink
      http://hdl.handle.net/11693/53550
      Published Version (Please cite this version)
      https://doi.org/10.1016/j.neuroscience.2008.01.004
      Collections
      • Aysel Sabuncu Brain Research Center (BAM) 183
      Show full item record

      Browse

      All of BUIRCommunities & CollectionsTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartmentsThis CollectionTitlesAuthorsAdvisorsBy Issue DateKeywordsTypeDepartments

      My Account

      Login

      Statistics

      View Usage StatisticsView Google Analytics Statistics

      Bilkent University

      If you have trouble accessing this page and need to request an alternate format, contact the site administrator. Phone: (312) 290 1771
      Copyright © Bilkent University - Library IT

      Contact Us | Send Feedback | Off-Campus Access | Admin | Privacy