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dc.contributor.authorAhan, Recep Erdemen_US
dc.contributor.authorKırpat, Büşra Merveen_US
dc.contributor.authorSaltepe, Behideen_US
dc.contributor.authorŞeker, Urartu Özgür Şafaken_US
dc.date.accessioned2020-02-12T08:14:32Z
dc.date.available2020-02-12T08:14:32Z
dc.date.issued2019
dc.identifier.issn2161-5063
dc.identifier.urihttp://hdl.handle.net/11693/53299
dc.description.abstractEngineered bacterial cells have great promise to solve global problems, yet they are hampered by a lack of convenient strategy for controlled protein release. A well-controlled protein translocation through cellular membranes is essential for cell-based protein delivery. Here we have developed a controlled protein release system by programming a bacterial autotransporter system named Ag43. Ag43 protein is engineered by adding a protease digestion site between its translocation and cargo domains. Once it is displayed on the cell surface, we managed to release the cargo proteins in defined conditions by processing environmental signals. The protein release in terms of time and quantity can be controlled through changing the inducer conditions. We thought that the release system can be adopted for complex genetic circuitries due to its simplicity. We implemented the protein release system to develop a cellular device that is able to release proteins in a sequence response to ordered chemical signals. We envision that development of genetically controlled protein release systems will improve the applications of synthetic organisms in cell based therapies, especially for cases with a need for controlled protein release using the cues from the biological environment.en_US
dc.language.isoEnglishen_US
dc.source.titleACS Synthetic Biologyen_US
dc.relation.isversionofhttps://dx.doi.org/10.1021/acssynbio.9b00062en_US
dc.subjectSequential secretionen_US
dc.subjectAutotransportersen_US
dc.subjectTEV proteaseen_US
dc.subjectProtein displayen_US
dc.titleA self-actuated cellular protein delivery machineen_US
dc.typeArticleen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.departmentInterdisciplinary Program in Neuroscience (NEUROSCIENCE)en_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.citation.spage686en_US
dc.citation.epage696en_US
dc.citation.volumeNumber8en_US
dc.citation.issueNumber4en_US
dc.identifier.doi10.1021/acssynbio.9b00062en_US
dc.publisherAmerican Chemical Societyen_US
dc.contributor.bilkentauthorAhan, Recep Erdem
dc.contributor.bilkentauthorKırpat, Büşra Merve
dc.contributor.bilkentauthorSaltepe, Behide
dc.contributor.bilkentauthorŞeker, Urartu Özgür Şafak


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