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dc.contributor.authorKalyoncu, Ebuzeren_US
dc.contributor.authorAhan, Recep Erdemen_US
dc.contributor.authorÖzçelik, Cemile Elifen_US
dc.contributor.authorŞeker, Urartu Özgür Şafaken_US
dc.date.accessioned2020-02-10T06:00:38Z
dc.date.available2020-02-10T06:00:38Z
dc.date.issued2019
dc.identifier.issn0935-9648
dc.identifier.urihttp://hdl.handle.net/11693/53201
dc.description.abstractDistinct spatial patterning of naturally produced materials is observed in many cellular structures and even among communities of microorganisms. Reoccurrence of spatially organized materials in all branches of life is clear proof that organization is beneficial for survival. Indeed, organisms can trick the evolutionary process by using organized materials in ways that can help the organism to avoid unexpected conditions. To expand the toolbox for synthesizing patterned living materials, Boolean type “AND” and “OR” control of curli fibers expression is demonstrated using recombinases. Logic gates are designed to activate the production of curli fibers. The gates can be used to record the presence of input molecules and give output as CsgA expression. Two different curli fibers (CsgA and CsgA‐His‐tag) production are then selectively activated to explore distribution of monomers upon coexpression. To keep track of the composition of fibers, CsgA‐His‐tag proteins are labeled with nickel–nitrilotriacetic acid (Ni–NTA‐) conjugated gold nanoparticles. It is observed that an organized living material can be obtained upon inducing the coexpression of different CsgA fibers. It is foreseen that living materials with user‐defined curli composition hold great potential for the development of living materials for many biomedical applications.en_US
dc.description.sponsorshipTUBITAK. Grant Numbers: 114M163, 216M127en_US
dc.language.isoEnglishen_US
dc.source.titleAdvanced Materialsen_US
dc.relation.isversionofhttps://doi.org/10.1002/adma.201902888en_US
dc.subjectAmyloid fibersen_US
dc.subjectBiofilm patterningen_US
dc.subjectGenetic circuitsen_US
dc.subjectGenetic logic gatesen_US
dc.subjectRecombinaseen_US
dc.titleGenetic logic gates enable patterning of amyloid nanofibersen_US
dc.typeArticleen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentInterdisciplinary Program in Neuroscience (NEUROSCIENCE)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.citation.spage1902888 (1)en_US
dc.citation.epage1902888 (7)en_US
dc.citation.volumeNumber31en_US
dc.citation.issueNumber39en_US
dc.identifier.doi10.1002/adma.201902888en_US
dc.publisherWILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.contributor.bilkentauthorKalyoncu, Ebuzer
dc.contributor.bilkentauthorAhan, Recep Erdem
dc.contributor.bilkentauthorÖzçelik, Cemile Elif
dc.contributor.bilkentauthorŞeker, Urartu Özgür Şafak
dc.embargo.release2020-09-23


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