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      Genetic logic gates enable patterning of amyloid nanofibers

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      Embargo Lift Date: 2020-09-23
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      Author(s)
      Kalyoncu, Ebuzer
      Ahan, Recep Erdem
      Özçelik, Cemile Elif
      Şeker, Urartu Özgür Şafak
      Date
      2019
      Source Title
      Advanced Materials
      Print ISSN
      0935-9648
      Publisher
      WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
      Volume
      31
      Issue
      39
      Pages
      1902888-1 - 1902888-7
      Language
      English
      Type
      Article
      Item Usage Stats
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      253
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      Abstract
      Distinct spatial patterning of naturally produced materials is observed in many cellular structures and even among communities of microorganisms. Reoccurrence of spatially organized materials in all branches of life is clear proof that organization is beneficial for survival. Indeed, organisms can trick the evolutionary process by using organized materials in ways that can help the organism to avoid unexpected conditions. To expand the toolbox for synthesizing patterned living materials, Boolean type “AND” and “OR” control of curli fibers expression is demonstrated using recombinases. Logic gates are designed to activate the production of curli fibers. The gates can be used to record the presence of input molecules and give output as CsgA expression. Two different curli fibers (CsgA and CsgA‐His‐tag) production are then selectively activated to explore distribution of monomers upon coexpression. To keep track of the composition of fibers, CsgA‐His‐tag proteins are labeled with nickel–nitrilotriacetic acid (Ni–NTA‐) conjugated gold nanoparticles. It is observed that an organized living material can be obtained upon inducing the coexpression of different CsgA fibers. It is foreseen that living materials with user‐defined curli composition hold great potential for the development of living materials for many biomedical applications.
      Keywords
      Amyloid fibers
      Biofilm patterning
      Genetic circuits
      Genetic logic gates
      Recombinase
      Permalink
      http://hdl.handle.net/11693/53201
      Published Version (Please cite this version)
      https://doi.org/10.1002/adma.201902888
      Collections
      • Aysel Sabuncu Brain Research Center (BAM) 249
      • Institute of Materials Science and Nanotechnology (UNAM) 2260
      • Nanotechnology Research Center (NANOTAM) 1179
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