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dc.contributor.authorGüçlüler, Gözdeen_US
dc.contributor.authorKahraman, Tameren_US
dc.contributor.authorGürsel, İhsanen_US
dc.coverage.spatialAntalya, Turkeyen_US
dc.descriptionConference Name: Molecular Immunology & Immunogenetics Congress, MIMIC 2012en_US
dc.descriptionDate of Conference: 27-29 April 2012en_US
dc.description.abstractExosomes are naturally occurring, membranous nanovesicles known to function as intercellular communication vectors. To explore whether these naturally occuring bilayer vesicles could be exploited as a nucleic acid delivery system we intentionally loaded exosomes with TLR9 ligands and studied their immunostimulatory properties. METHODS: Exosomes were isolated from RAW264.7 and EG-7 cell supernatants by differential centrifugation, filtration and ultracentrifugation and were loaded with CpG ODNs via dehydration-rehydration protocol. Splenocytes were stimulated and analyzed by flow cytometry for cell surface marker upregulation, intracellular cytokine production and co-stimulatory molecule upregulation. Confocal microscopy analyses were done to assess internalization properties of these nanovesciles. Cell supernatants were studied by ELISA to assess Th1 biased cytokine/chemokine secretion.en_US
dc.source.titleMolecular Immunology & Immunogenetics Congress, MIMIC 2012en_US
dc.subjectTLR liganden_US
dc.subjectImmune stimulationen_US
dc.titleCpG ODN loaded exosome nanovesicles: enhanced immunostimulatory activityen_US
dc.typeConference Paperen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.publisherTurkish Society of Immunologyen_US

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