CpG ODN loaded exosome nanovesicles: enhanced immunostimulatory activity
Molecular Immunology & Immunogenetics Congress, MIMIC 2012
Turkish Society of Immunology
Item Usage Stats
MetadataShow full item record
Exosomes are naturally occurring, membranous nanovesicles known to function as intercellular communication vectors. To explore whether these naturally occuring bilayer vesicles could be exploited as a nucleic acid delivery system we intentionally loaded exosomes with TLR9 ligands and studied their immunostimulatory properties. METHODS: Exosomes were isolated from RAW264.7 and EG-7 cell supernatants by differential centrifugation, filtration and ultracentrifugation and were loaded with CpG ODNs via dehydration-rehydration protocol. Splenocytes were stimulated and analyzed by flow cytometry for cell surface marker upregulation, intracellular cytokine production and co-stimulatory molecule upregulation. Confocal microscopy analyses were done to assess internalization properties of these nanovesciles. Cell supernatants were studied by ELISA to assess Th1 biased cytokine/chemokine secretion.
- Work in Progress 349