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dc.contributor.authorYuluğ, Işıken_US
dc.contributor.authorAtalay, A.en_US
dc.contributor.editorHayat, M.
dc.date.accessioned2019-05-07T13:26:52Z
dc.date.available2019-05-07T13:26:52Z
dc.date.issued2004en_US
dc.identifier.urihttp://hdl.handle.net/11693/51149
dc.descriptionChapter 10en_US
dc.description.abstractThis chapter describes suppression subtractive hybridization (SSH)—a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. SSH accomplishes normalization and subtraction by taking advantage of the different rates of hybridization of cDNA strands for different genes depending on their abundance level and the degree of (differential) expression. SSH may be used for pairwise treatment comparisons and must be replicated with the tester and driver reversed to identify gene expression changes in both directions. It is not a quantitative method for measuring expression differences. SSH is best used for identifying genes that are completely absent, rather than expressed less abundantly, in the driver sample. One of the major problems associated with specific cellular characterization is the low amount of sample. However, problems associated with tissues in small quantities can be solved by a restricted polymerase chain reaction (PCR) amplification step prior to cDNA subtraction. When the amount of starting material is limited, it is possible to start with only a few nanograms of total RNA and produce enough double-stranded cDNA of both tester and driver to subtract two specific cell populations by using PCR technology.en_US
dc.language.isoEnglishen_US
dc.relation.ispartofHandbook of immunohistochemistry and in situ hybridization of human carcinomas: molecular pathology, colorectal carcinoma, prostate carcinomaen_US
dc.relation.isversionofhttps://doi.org/10.1016/S1874-5784(02)80016-2en_US
dc.titleSuppression subtractive hybridization technologyen_US
dc.typeBook Chapteren_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage113en_US
dc.citation.epage126en_US
dc.citation.volumeNumber2en_US
dc.identifier.doi10.1016/S1874-5784(02)80016-2en_US
dc.publisherElsevieren_US
dc.identifier.eisbn9780123339423


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