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dc.contributor.authorSever, Melikeen_US
dc.contributor.authorGunay, Gokhanen_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, Ayse B.en_US
dc.date.accessioned2019-02-21T16:02:41Z
dc.date.available2019-02-21T16:02:41Z
dc.date.issued2018en_US
dc.identifier.issn2047-4830
dc.identifier.urihttp://hdl.handle.net/11693/50032
dc.description.abstractThe development of new biomaterials mimicking the neuronal extracellular matrix (ECM) requires signals for the induction of neuronal differentiation and regeneration. In addition to the biological and chemical cues, the physical properties of the ECM should also be considered while designing regenerative materials for nervous tissue. In this study, we investigated the influence of the microenvironment on tenascin-C signaling using 2D surfaces and 3D scaffolds generated by a peptide amphiphile nanofiber gel with a tenascin-C derived peptide epitope (VFDNFVLK). While tenascin-C mimetic PA nanofibers significantly increased the length and number of neurites produced by PC12 cells on 2D cell culture, more extensive neurite outgrowth was observed in the 3D gel environment. PC12 cells encapsulated within the 3D tenascin-C mimetic peptide nanofiber gel also exhibited significantly increased expression of neural markers compared to the cells on 2D surfaces. Our results emphasize the synergistic effects of the 3D conformation of peptide nanofibers along with the tenascin-C signaling and growth factors on the neuronal differentiation of PC12 cells, which may further provide more tissue-like morphology for therapeutic applications.
dc.description.sponsorshipWe thank Z. Erdogan and M. Guler for their technical help in the purification and characterization of PA nanofibers. M. S. is supported by the TUBITAK-BIDEB (2211) Ph.D. fellowship, and G. G. is supported by the TUBITAK-BIDEB (2210-C) M.Sc. fellowship. A. B. T. acknowledges the support from the Science Academy Outstanding Young Scientist Award (BAGEP).
dc.language.isoEnglish
dc.source.titleBiomaterials Scienceen_US
dc.relation.isversionofhttps://doi.org/10.1039/c7bm00850c
dc.titleTenascin-C derived signaling induces neuronal differentiation in a three-dimensional peptide nanofiber gelen_US
dc.typeArticleen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.departmentInterdisciplinary Program in Neuroscience (NEUROSCIENCE)en_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.citation.spage1859en_US
dc.citation.epage1868en_US
dc.citation.volumeNumber6en_US
dc.citation.issueNumber7en_US
dc.relation.project2210-C
dc.identifier.doi10.1039/c7bm00850c
dc.publisherRoyal Society of Chemistry
dc.contributor.bilkentauthorSever, Melike
dc.contributor.bilkentauthorGunay, Gokhan
dc.contributor.bilkentauthorGüler, Mustafa O.
dc.contributor.bilkentauthorTekinay, Ayse B.


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