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dc.contributor.authorCalienni, M. N.en_US
dc.contributor.authorTemprana, C. F.en_US
dc.contributor.authorPrieto, M. J.en_US
dc.contributor.authorPaolino, D.en_US
dc.contributor.authorFresta, M.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.contributor.authordel, Valle, Alonso, S.en_US
dc.contributor.authorMontanari, J.en_US
dc.date.accessioned2019-02-21T16:01:20Zen_US
dc.date.available2019-02-21T16:01:20Zen_US
dc.date.issued2018en_US
dc.identifier.issn2190-393Xen_US
dc.identifier.urihttp://hdl.handle.net/11693/49821en_US
dc.description.abstractWith the aim of improving the topical delivery of the antineoplastic drug 5-fluorouracil (5FU), it was loaded into ultradeformable liposomes composed of soy phosphatidylcholine and sodium cholate (UDL-5FU). The liposome populations had a mean size of 70 nm without significant changes in 56 days, and the ultradeformable formulations were up to 324-fold more elastic than conventional liposomes. The interaction between 5FU and the liposomal membrane was studied by three methods, and also release profile was obtained. UDL-5FU did penetrate the stratum corneum of human skin. At in vitro experiments, the formulationwas more toxic on a human melanoma-derived than on a human keratinocyte-derived cell line. Cells captured liposomes by metabolically active processes. In vivo toxicity experiments were carried out in zebrafish (Danio rerio) larvae by studying the swimming activity, morphological changes, and alterations in the heart rate after incubation. UDL-5FU was more toxic than free 5FU. Therefore, this nano-formulation could be useful for topical application in deep skin precancerous lesions with advantages over current treatments. This is the first work that assessed the induction of apoptosis, skin penetration in a Saarbrücken penetration model, and the toxicological effects in vivo of an ultradeformable 5FU-loaded formulation.en_US
dc.description.sponsorshipthe blueberry, and Dr. Juan Montes de Oca and Dr. Roberto Candal of INQUIMAE-CONICET. Funding information This work was supported by a grant from Universidad Nacional de Quilmes and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (PIP 0358, 2014-2016).en_US
dc.language.isoEnglishen_US
dc.source.titleDrug Delivery and Translational Researchen_US
dc.relation.isversionofhttps://doi.org/10.1007/s13346-017-0469-1en_US
dc.subject5-Fluorouracilen_US
dc.subjectSkin therapyen_US
dc.subjectTopical deliveryen_US
dc.subjectUltradeformable liposomesen_US
dc.subjectZebrafishen_US
dc.titleNano-formulation for topical treatment of precancerous lesions: Skin penetration, in vitro, and in vivo toxicological evaluationen_US
dc.typeArticleen_US
dc.departmentUNAM - Institute of Materials Science and Nanotechnologyen_US
dc.departmentNANOTAM - Nanotechnology Research Centeren_US
dc.citation.spage496en_US
dc.citation.epage514en_US
dc.citation.volumeNumber8en_US
dc.citation.issueNumber3en_US
dc.relation.projectMinistero degli Affari Esteri e della Cooperazione Internazionale, MAECI - Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET - Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET - Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET: PIP 0358en_US
dc.identifier.doi10.1007/s13346-017-0469-1en_US
dc.publisherSpringer Verlagen_US
dc.identifier.eissn2190-3948en_US


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