dc.contributor.advisor | Hanay, Mehmet Selim | |
dc.contributor.author | Orhan, Ezgi | |
dc.date.accessioned | 2018-08-28T06:18:33Z | |
dc.date.available | 2018-08-28T06:18:33Z | |
dc.date.copyright | 2018-08 | |
dc.date.issued | 2018-08 | |
dc.date.submitted | 2018-08-15 | |
dc.identifier.uri | http://hdl.handle.net/11693/47748 | |
dc.description | Cataloged from PDF version of article. | en_US |
dc.description | Thesis (M.S.): Bilkent University, Department of Mechanical, İhsan Doğramacı Bilkent University, 2018. | en_US |
dc.description | Includes bibliographical references (leaves 61-65). | en_US |
dc.description.abstract | Mass spectrometry (MS) is a technique used frequently in mass measurements
in order to identify mass of the molecules. Nanoelectromechanical systems are
highly sensitive to adhered species, thus using NEMS devices, it is possible to
perform NEMS-MS where not only the inertial mass of the molecules but also
the position of the adhered particle can be found out by resolving the adsorbateinduced
frequency shifts in the first two modes.By using frequency shifts obtained
from three mechanical modes, it is possible to obtain stiffness of the adsorbate in
addition to its mass and position on the resonator when the Youngs modulus of
the analyte and the resonant structure are comparable. For soft analytes, multimode
information can be used to obtain shape properties of analytes and allows
for image reconstruction from global image features. In order to conduct our
experiments, we fabricate NEMS resonators whose transduction method is electrothermal
actuation and piezoresistive detection. Fabrications of the devices are
completed in National Nanotechnology Research Center (UNAM) in Bilkent University
and Sabancı University Nanotechnology Research and Application Center
(SUNUM).Initially, low vacuum apparatus is built to perform NEMS-MS using
Electrospray Ionization(ESI) for molecule delivery. In order to direct particles to
resonator, the fabrication of a doubly clamped beam is planned in a way that the
orifice was etched through silicon wafer from the backside with KOH etch.This
fabrication method, however, is tedious and hard to fabricate consistently.Then,
Matrix Assisted Laser Desorption and Ionization (MALDI) is implemented to
deliver particles towards the resonator.Different analyte types which are gold
nanoparticles, centrosome organelles of HeLa cells and M13ke bacteriophages
are used in the experiments.We use first four out-of-plane modes of the doublyclamped
beam resonator for real-time study of the adsorbates. For biomolecule
detection, care was taken to prevent uniform coverage of matrix molecules. Phaselocked-loop(PLL)
operation is simultaneously performed for the first four modes
of the resonator.Using frequency shifts of the four modes due to the adsorption,
we propose a method in which we assume the analytes adhered on the beam are
hemispherical to obtain mass and stiffness, size and positions of the analytes.
Using three mechanical modes, stiffness, mass and position vaues are calculated. | en_US |
dc.description.statementofresponsibility | by Ezgi Orhan. | en_US |
dc.format.extent | xv, 72 leaves : illustrations (some color), charts ; 30 cm. | en_US |
dc.language.iso | English | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | NEMS | en_US |
dc.subject | Nanofabrication | en_US |
dc.subject | Electrothermal Actuation | en_US |
dc.subject | Piezoresistive Detection | en_US |
dc.subject | Multimode Mass Sensing | en_US |
dc.subject | Mass Spectrometry | en_US |
dc.subject | Stiffness Spectrometry | en_US |
dc.subject | Electrospray Ionization | en_US |
dc.subject | Matrix Assisted Laser Desorption and Ionization | en_US |
dc.title | Mass and stiffness spectrometry of nanoparticles and bio-molecules by nanoelectromechanical systems | en_US |
dc.title.alternative | Nanoparçacık ve biomoleküllerin kütle ve sertliklerinin nanoelektromekanik sistemler ile ölçülmesi | en_US |
dc.type | Thesis | en_US |
dc.department | Department of Mechanical Engineering | en_US |
dc.publisher | Bilkent University | en_US |
dc.description.degree | M.S. | en_US |
dc.identifier.itemid | B158773 | |
dc.embargo.release | 2019-08-09 | |