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      Atomic force microscopy for the investigation of molecular and cellular behavior

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      Author(s)
      Ozkan A.D.
      Topal, A. E.
      Dana, A.
      Güler, Mustafa O.
      Tekinay, A. B.
      Date
      2016-10
      Source Title
      Micron
      Print ISSN
      0968-4328
      Publisher
      Elsevier
      Volume
      89
      Pages
      60 - 76
      Language
      English
      Type
      Review
      Item Usage Stats
      241
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      310
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      Abstract
      The present review details the methods used for the measurement of cells and their exudates using atomic force microscopy (AFM) and outlines the general conclusions drawn by the mechanical characterization of biological materials through this method. AFM is a material characterization technique that can be operated in liquid conditions, allowing its use for the investigation of the mechanical properties of biological materials in their native environments. AFM has been used for the mechanical investigation of proteins, nucleic acids, biofilms, secretions, membrane bilayers, tissues and bacterial or eukaryotic cells; however, comparison between studies is difficult due to variances between tip sizes and morphologies, sample fixation and immobilization strategies, conditions of measurement and the mechanical parameters used for the quantification of biomaterial response. Although standard protocols for the AFM investigation of biological materials are limited and minor differences in measurement conditions may create large discrepancies, the method is nonetheless highly effective for comparatively evaluating the mechanical integrity of biomaterials and can be used for the real-time acquisition of elasticity data following the introduction of a chemical or mechanical stimulus. While it is currently of limited diagnostic value, the technique is also useful for basic research in cancer biology and the characterization of disease progression and wound healing processes.
      Keywords
      Atomic force microscopy
      Biomacromolecules
      Cells
      Mechanical characterization
      Atomic force microscopy
      Biological materials
      Biomaterials
      Biomechanics
      Cells
      Diagnosis
      Nucleic acids
      Biomacromolecules
      Material characterization techniques
      Measurement conditions
      Mechanical characterizations
      Mechanical integrity
      Mechanical parameters
      Real time acquisition
      Wound healing process
      Characterization
      Biomaterial
      Protein
      Atomic force microscopy
      Bacterial phenomena and functions
      Bacterium
      Biofilm
      Cell function
      Elasticity
      Human
      Procedures
      Ultrastructure
      Bacteria
      Bacterial Physiological Phenomena
      Biocompatible Materials
      Biofilms
      Cell Physiological Phenomena
      Elasticity
      Humans
      Microscopy, Atomic Force
      Proteins
      Permalink
      http://hdl.handle.net/11693/38129
      Published Version (Please cite this version)
      https://doi.org/10.1016/j.micron.2016.07.011
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      • Institute of Materials Science and Nanotechnology (UNAM) 2098
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