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      Detection of Calcium-induced morphological changes on RBCs by digital holographic microscopy and blinking optical tweezers

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      Author(s)
      Rad, V. F.
      Tavakkoli, R.
      Moradi, Ali-Reza
      Anand, A.
      Javidi, B.
      Date
      2016
      Source Title
      Proceedings of the 15th Workshop on Information Optics, WIO 2016
      Publisher
      IEEE
      Pages
      1 - 3
      Language
      English
      Type
      Conference Paper
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      Abstract
      Ca+2 level in the circulating red blood cells (RBCs) takes part not only in controlling biophysical properties, but also affects the membrane composition, and its morphological and rheological properties. Excessive accumulation of Ca2+ within the cells is associated with a number of important pathological diseases. In this paper, by the use of digital holographic microscopy (DHM), we quantitatively analyzed the volumetric behavior of RBC membrane under influence of excess Calcium ions. DHM in a transmission mode is an effective tool for quantitative visualization of phase objects. By deriving the associated phase changes 3D information on the morphology variation of the cells at arbitrary time scales is obtained. Individual cells are immobilized by the use of optical tweezers and are monitored live with DHM system, while the concentration of Ca2+ ions in the buffer is changed simultaneously. We utilized blinking optical tweezers, by inserting an optical chopper to modulate intensity of the trapping laser beam. Blinking optical tweezers, while keeping the cell trapped during the experiments, ensures of minimizing the photo-damage of trapping laser beam on the cell. Our experimental results are in agreement with previous biological studies and predictions, and experimental observations of living RBCs under Ca2+ influence.
      Keywords
      Calcium
      Cells
      Cytology
      Holography
      Ions
      Laser beams
      Microscopic examination
      Phase measurement
      Biophysical properties
      Digital holographic microscopy
      Membrane composition
      Morphological changes
      Pathological disease
      Quantitative visualization
      Rheological property
      Volumetric behavior
      Optical tweezers
      Permalink
      http://hdl.handle.net/11693/37683
      Published Version (Please cite this version)
      http://dx.doi.org/10.1109/WIO.2016.7745588
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