Femtosecond laser fabrication of fiber based optofluidic platform for flow cytometry applications
Date
2017Source Title
Proceedings of SPIE, Progress in Biomedical Optics and Imaging
Print ISSN
1605-7422
Publisher
SPIE
Volume
10058
Language
English
Type
Conference PaperItem Usage Stats
261
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views
215
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Abstract
Miniaturized optofluidic platforms play an important role in bio-analysis, detection and diagnostic applications. The advantages of such miniaturized devices are extremely low sample requirement, low cost development and rapid analysis capabilities. Fused silica is advantageous for optofluidic systems due to properties such as being chemically inert, mechanically stable, and optically transparent to a wide spectrum of light. As a three dimensional manufacturing method, femtosecond laser scanning followed by chemical etching shows great potential to fabricate glass based optofluidic chips. In this study, we demonstrate fabrication of all-fiber based, optofluidic flow cytometer in fused silica glass by femtosecond laser machining. 3D particle focusing was achieved through a straightforward planar chip design with two separately fabricated fused silica glass slides thermally bonded together. Bioparticles in a fluid stream encounter with optical interrogation region specifically designed to allocate 405nm single mode fiber laser source and two multi-mode collection fibers for forward scattering (FSC) and side scattering (SSC) signals detection. Detected signal data collected with oscilloscope and post processed with MATLAB script file. We were able to count number of events over 4000events/sec, and achieve size distribution for 5.95μm monodisperse polystyrene beads using FSC and SSC signals. Our platform shows promise for optical and fluidic miniaturization of flow cytometry systems. © 2017 SPIE.
Keywords
All-fiberFemtosecond laser
Flow cytometry
Fused silica
Hydrodynamic focusing
Machining
Microfluidics
Optofluidic
Bioassay
Biochips
Diagnosis
Emission spectroscopy
Fabrication
Fiber lasers
Fibers
Flow cytometry
Forward scattering
Glass
Machining
Microarrays
Microfluidics
Miniature instruments
Optical fiber fabrication
Optical fibers
Signal detection
Silica
Single mode fibers
Textile fibers
Ultrashort pulses
All fiber
Detection and diagnostics
Femtosecond laser machining
Flow cytometry systems
Hydrodynamic focusing
Monodisperse polystyrene
Optofluidic
Three-dimensional manufacturing
Fused silica
Permalink
http://hdl.handle.net/11693/37566Published Version (Please cite this version)
https://doi.org/10.1117/12.2252092Collections
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