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      Femtosecond laser fabrication of fiber based optofluidic platform for flow cytometry applications

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      Author(s)
      Serhatlioglu, Murat
      Elbuken, Çağlar
      Ortac, Bülend
      Solmaz, Mehmet E.
      Date
      2017
      Source Title
      Proceedings of SPIE, Progress in Biomedical Optics and Imaging
      Print ISSN
      1605-7422
      Publisher
      SPIE
      Volume
      10058
      Language
      English
      Type
      Conference Paper
      Item Usage Stats
      261
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      215
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      Abstract
      Miniaturized optofluidic platforms play an important role in bio-analysis, detection and diagnostic applications. The advantages of such miniaturized devices are extremely low sample requirement, low cost development and rapid analysis capabilities. Fused silica is advantageous for optofluidic systems due to properties such as being chemically inert, mechanically stable, and optically transparent to a wide spectrum of light. As a three dimensional manufacturing method, femtosecond laser scanning followed by chemical etching shows great potential to fabricate glass based optofluidic chips. In this study, we demonstrate fabrication of all-fiber based, optofluidic flow cytometer in fused silica glass by femtosecond laser machining. 3D particle focusing was achieved through a straightforward planar chip design with two separately fabricated fused silica glass slides thermally bonded together. Bioparticles in a fluid stream encounter with optical interrogation region specifically designed to allocate 405nm single mode fiber laser source and two multi-mode collection fibers for forward scattering (FSC) and side scattering (SSC) signals detection. Detected signal data collected with oscilloscope and post processed with MATLAB script file. We were able to count number of events over 4000events/sec, and achieve size distribution for 5.95μm monodisperse polystyrene beads using FSC and SSC signals. Our platform shows promise for optical and fluidic miniaturization of flow cytometry systems. © 2017 SPIE.
      Keywords
      All-fiber
      Femtosecond laser
      Flow cytometry
      Fused silica
      Hydrodynamic focusing
      Machining
      Microfluidics
      Optofluidic
      Bioassay
      Biochips
      Diagnosis
      Emission spectroscopy
      Fabrication
      Fiber lasers
      Fibers
      Flow cytometry
      Forward scattering
      Glass
      Machining
      Microarrays
      Microfluidics
      Miniature instruments
      Optical fiber fabrication
      Optical fibers
      Signal detection
      Silica
      Single mode fibers
      Textile fibers
      Ultrashort pulses
      All fiber
      Detection and diagnostics
      Femtosecond laser machining
      Flow cytometry systems
      Hydrodynamic focusing
      Monodisperse polystyrene
      Optofluidic
      Three-dimensional manufacturing
      Fused silica
      Permalink
      http://hdl.handle.net/11693/37566
      Published Version (Please cite this version)
      https://doi.org/10.1117/12.2252092
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      • Institute of Materials Science and Nanotechnology (UNAM) 2098
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