Angiogenic peptide nanofibers repair cardiac tissue defect after myocardial infarction
Author
Rufaihah, A. J.
Yasa, I. C.
Ramanujam, V. S.
Arularasu, S. C.
Kofidis, T.
Güler, Mustafa O.
Tekinay, A. B.
Date
2017Source Title
Acta Biomaterialia
Print ISSN
1742-7061
Electronic ISSN
1878-7568
Publisher
Acta Materialia Inc
Volume
58
Pages
102 - 112
Language
English
Type
ArticleItem Usage Stats
175
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views
205
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downloads
Abstract
Myocardial infarction remains one of the top leading causes of death in the world and the damage sustained in the heart eventually develops into heart failure. Limited conventional treatment options due to the inability of the myocardium to regenerate after injury and shortage of organ donors require the development of alternative therapies to repair the damaged myocardium. Current efforts in repairing damage after myocardial infarction concentrates on using biologically derived molecules such as growth factors or stem cells, which carry risks of serious side effects including the formation of teratomas. Here, we demonstrate that synthetic glycosaminoglycan (GAG) mimetic peptide nanofiber scaffolds induce neovascularization in cardiovascular tissue after myocardial infarction, without the addition of any biologically derived factors or stem cells. When the GAG mimetic nanofiber gels were injected in the infarct site of rodent myocardial infarct model, increased VEGF-A expression and recruitment of vascular cells was observed. This was accompanied with significant degree of neovascularization and better cardiac performance when compared to the control saline group. The results demonstrate the potential of future clinical applications of these bioactive peptide nanofibers as a promising strategy for cardiovascular repair. Statement of Significance We present a synthetic bioactive peptide nanofiber system can enhance cardiac function and enhance cardiovascular regeneration after myocardial infarction (MI) without the addition of growth factors, stem cells or other biologically derived molecules. Current state of the art in cardiac repair after MI utilize at least one of the above mentioned biologically derived molecules, thus our approach is ground-breaking for cardiovascular therapy after MI. In this work, we showed that synthetic glycosaminoglycan (GAG) mimetic peptide nanofiber scaffolds induce neovascularization and cardiomyocyte differentiation for the regeneration of cardiovascular tissue after myocardial infarction in a rat infarct model. When the peptide nanofiber gels were injected in infarct site at rodent myocardial infarct model, recruitment of vascular cells was observed, neovascularization was significantly induced and cardiac performance was improved. These results demonstrate the potential of future clinical applications of these bioactive peptide nanofibers as a promising strategy for cardiovascular repair.
Keywords
CardiomyocyteMyocardial infarction
Neovascularization
Peptide nanofibers
VEGF
Alpha smooth muscle actin
Fibroblast growth factor 2
Glycosaminoglycan
Molecular scaffold
Nanofiber
Scatter factor
Sodium chloride
Troponin T
Vasculotropin A
Angiogenic factor
Nanofiber
Peptide
Vascular endothelial growth factor A, rat
Vasculotropin A
Animal cell
Animal experiment
Animal model
Article
Cell adhesion
Cell differentiation
Cell fate
Controlled study
Heart function
Heart infarction
Heart left ventricle pressure
Heart muscle contractility
Heart output
Heart performance
Heart tissue
Male
Neovascularization (pathology)
Nonhuman
Priority journal
Protein expression
Rat
Stem cell
Animal
Biosynthesis
Cardiac muscle
Chemistry
Disease model
Heart infarction
Metabolism
Pathology
Wistar rat
Angiogenesis inducing agents
Animals
Disease models, animal
Male
Myocardial infarction
Myocardium
Nanofibers
Peptides
Rats
Rats, wistar
Vascular endothelial growth factor A
Permalink
http://hdl.handle.net/11693/37443Published Version (Please cite this version)
http://dx.doi.org/10.1016/j.actbio.2017.06.009Collections
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