Heparin mimetic peptide nanofiber gel promotes regeneration of full thickness burn injury
Embargo Lift Date: 2019-07-01
117 - 127
Item Usage Stats
Burn injuries are one of the most common types of trauma worldwide, and their unique physiology requires the development of specialized therapeutic materials for their treatment. Here, we report the use of synthetic, functional and biodegradable peptide nanofiber gels for the improved healing of burn wounds to alleviate the progressive loss of tissue function at the post-burn wound site. These bioactive nanofiber gels form scaffolds that recapitulate the structure and function of the native extracellular matrix through signaling peptide epitopes, which can trigger angiogenesis through their affinity to basic growth factors. In this study, the angiogenesis-promoting properties of the bioactive scaffolds were utilized for the treatment of a thermal burn model. Following the excision of necrotic tissue, bioactive gels and control solutions were applied topically onto the wound area. The wound healing process was evaluated at 7, 14 and 21 days following injury through histological observations, immunostaining and marker RNA/protein analysis. Bioactive peptide nanofiber-treated burn wounds formed well-organized and collagen-rich granulation tissue layers, produced a greater density of newly formed blood vessels, and exhibited increased re-epithelialization and skin appendage development with minimal crust formation, while non-bioactive peptide nanofibers and the commercial wound dressing 3M™ Tegaderm™ did not exhibit significant efficiency over sucrose controls. Overall, the heparin-mimetic peptide nanofiber gels increased the rate of repair of burn injuries and can be used as an effective means of facilitating wound healing.
High temperature effects
Native extracellular matrix
Wound healing process
Full thickness skin graft
In vivo study
Bagg albino mouse
Scanning electron microscopy
Mice, inbred BALB C
Microscopy, electron, scanning
Published Version (Please cite this version)http://dx.doi.org/10.1016/j.biomaterials.2017.04.040
Showing items related by title, author, creator and subject.
Uzunalli, G.; Mammadov R.; Yesildal, F.; Alhan, D.; Ozturk, S.; Ozgurtas, T.; Güler, Mustafa O.; Tekinay, A. B. (American Chemical Society, 2017)Wound repair in adult mammals typically ends with the formation of a scar, which prevents full restoration of the function of the healthy tissue, although most of the wounded skin heals. Rapid and functional recovery of ...
Tansik, G.; Kilic, E.; Beter, M.; Demiralp, B.; K.Sendur, G.; Can, N.; Ozkan, H.; Ergul, E.; Güler, Mustafa O.; Tekinay, A. B. (Royal Society of Chemistry, 2016)Biomineralization of the extracellular matrix (ECM) plays a crucial role in bone formation. Functional and structural biomimetic native bone ECM components can therefore be used to change the fate of stem cells and induce ...
Mammadov, R.; Duman, E.; Güler, Mustafa O.; Tekinay, A. B. (Nova Science Publishers, Inc., 2013)Albeit the roles of growth factors (e.g. VEGF) and their receptors in angiogenesis have been emphasized extensively, the indispensable role of glycosaminoglycans, especially heparan sulfates, has been discerning recently. ...