Controlled enzymatic stability and release characteristics of supramolecular chiral peptide amphiphile nanofiber gels
Date
2017Source Title
Current Applied Physics
Print ISSN
1567-1739
Electronic ISSN
1878-1675
Publisher
Elsevier B.V.
Volume
17
Issue
5
Pages
785 - 792
Language
English
Type
ArticleItem Usage Stats
208
views
views
244
downloads
downloads
Abstract
Supramolecular bioarchitectures formed by assembly of achiral or chiral building blocks play important roles in various biochemical processes. Stereochemistry of amino acids is important for structural organization of peptide and protein assemblies and structure-microenvironment interactions. In this study, oppositely charged peptide amphiphile (PA) molecules with L-, D- and mixture of L- and D-amino acid conformations are coassembled into supramolecular nanofibers and formed self-supporting gels at pH 7.4 in water. The enzymatic stability of the PA nanofiber gels was studied in the presence of proteinase K enzyme, which digest a broad spectrum of proteins and peptides. The structural changes on the chiral PA nanofibers were also analyzed at different time periods in the presence of enzymatic activity. Controlled release of a model cargo molecule through the chiral PA nanofiber gels was monitored. The diffusivity parameters were measured for all gel systems. Release characteristics and the enzymatic stability of the peptide nanofiber gels were modulated depending on organization of the chiral PA molecules within the supramolecular assemblies.
Keywords
Controlled releaseEnzymatic stability
Nanofibers
Peptide amphiphile
Self-assembly
Supramolecular chirality
Amino acids
Amphiphiles
Enzyme activity
Gels
Molecules
Nanofibers
Peptides
Proteins
Self assembly
Stability
Supramolecular chemistry
Chiral building blocks
Controlled release
Enzymatic stability
Peptide amphiphiles
Release characteristics
Structural organization
Supramolecular assemblies
Supramolecular chirality
Stereochemistry
Permalink
http://hdl.handle.net/11693/37431Published Version (Please cite this version)
http://dx.doi.org/10.1016/j.cap.2017.02.026Collections
Related items
Showing items related by title, author, creator and subject.
-
Supramolecular assemblies constructed by cucurbituril-catalyzed click reaction
Tuncel, D.; Ünal, Ö.; Artar, M. (2011)Cucurbituril homologues are multi-functional macrocycles that can find applications in many areas and have numerous interesting features setting them apart from the other macrocycles. Among them, the ability of one of ... -
Interfiber interactions alter the stiffness of gels formed by supramolecular self-assembled nanofibers
Dagdas, Y. S.; Tombuloglu, A.; Tekinay, A. B.; Dana, A.; Güler, Mustafa O. (Elsevier, 2011-02-16)Molecular self-assembly is a powerful technique for developing novel nanostructures by using noncovalent interactions such as hydrogen bonding, hydrophobic, electrostatic, metal-ligand, p-p and van der Waals interactions. ... -
Biocompatible supramolecular catalytic one-dimensional nanofibers for efficient labeling of live cells
Khalily, M. A.; Gulseren, G.; Tekinay, A. B.; Güler, Mustafa O. (American Chemical Society, 2015)Understanding complex cellular functions requires study and tracking of biomolecules such as proteins, glycans, and lipids in their natural environment. Herein, we report the first supramolecular nanocatalyst for bioorthogonal ...