Aging alters the molecular dynamics of synapses in a sexually dimorphic pattern in zebrafish (Danio rerio)
Author(s)
Date
2017-06Source Title
Neurobiology of Aging
Print ISSN
0197-4580
Publisher
Elsevier
Volume
54
Pages
10 - 21
Language
English
Type
ArticleItem Usage Stats
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344
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Abstract
The zebrafish has become a popular model for studying normal brain aging due to its large fecundity, conserved genome, and available genetic tools; but little data exists about neurobiological age-related alterations. The current study tested the hypothesis of an association between brain aging and synaptic protein loss across males and females. Western blot analysis of synaptophysin (SYP), a presynaptic vesicle protein, and postsynaptic density-95 (PSD-95) and gephyrin (GEP), excitatory and inhibitory postsynaptic receptor-clustering proteins, respectively, was performed in young, middle-aged, and old male and female zebrafish (Danio rerio) brains. Univariate and multivariate analyses demonstrated that PSD-95 significantly increased in aged females and SYP significantly decreased in males, but GEP was stable. Thus, these key synaptic proteins vary across age in a sexually dimorphic manner, which has been observed in other species, and these consequences may represent selective vulnerabilities for aged males and females. These data expand our knowledge of normal aging in zebrafish, as well as further establish this model as an appropriate one for examining human brain aging.
Keywords
AgingExcitatory synapses
Gender
Inhibitory synapses
Synaptic proteins
Zebrafish
Beta tubulin
Gephyrin
Postsynaptic density protein 95
Synaptophysin
Carrier protein
Gephyrin
Membrane protein
SAP90/PSD95 associated protein
synaptophysin
Adult
Aged
Animal tissue
Brain level
Controlled study
Juvenile animal
Nonhuman
Protein analysis
Protein depletion
Protein expression
Protein function
Protein localization
Sequence homology
Sex difference
Synaptic transmission
Western blotting
Zebra fish
Analysis of variance
Animal
Animal model
Brain
Genetics
Human
Metabolism
Physiology
Sexual characteristics
Synapse
Brain
Carrier Proteins
SAP90-PSD95 Associated Proteins
Sex Characteristics
Synapses
Synaptophysin
Permalink
http://hdl.handle.net/11693/37350Published Version (Please cite this version)
https://doi.org/10.1016/j.neurobiolaging.2017.02.007Collections
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