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      Aging alters the molecular dynamics of synapses in a sexually dimorphic pattern in zebrafish (Danio rerio)

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      Author
      Karoglu, Elif Tugce
      Halim, Dilara Ozge
      Erkaya, Bahriye
      Altaytas, Ferda
      Arslan-Ergul, Ayca
      Konu, Ozlen
      Adams, Michelle M.
      Date
      2017-06
      Source Title
      Neurobiology of Aging
      Print ISSN
      0197-4580
      Publisher
      Elsevier
      Volume
      54
      Pages
      10 - 21
      Language
      English
      Type
      Article
      Item Usage Stats
      195
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      200
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      Abstract
      The zebrafish has become a popular model for studying normal brain aging due to its large fecundity, conserved genome, and available genetic tools; but little data exists about neurobiological age-related alterations. The current study tested the hypothesis of an association between brain aging and synaptic protein loss across males and females. Western blot analysis of synaptophysin (SYP), a presynaptic vesicle protein, and postsynaptic density-95 (PSD-95) and gephyrin (GEP), excitatory and inhibitory postsynaptic receptor-clustering proteins, respectively, was performed in young, middle-aged, and old male and female zebrafish (Danio rerio) brains. Univariate and multivariate analyses demonstrated that PSD-95 significantly increased in aged females and SYP significantly decreased in males, but GEP was stable. Thus, these key synaptic proteins vary across age in a sexually dimorphic manner, which has been observed in other species, and these consequences may represent selective vulnerabilities for aged males and females. These data expand our knowledge of normal aging in zebrafish, as well as further establish this model as an appropriate one for examining human brain aging.
      Keywords
      Aging
      Excitatory synapses
      Gender
      Inhibitory synapses
      Synaptic proteins
      Zebrafish
      Beta tubulin
      Gephyrin
      Postsynaptic density protein 95
      Synaptophysin
      Carrier protein
      Gephyrin
      Membrane protein
      SAP90/PSD95 associated protein
      synaptophysin
      Adult
      Aged
      Animal tissue
      Brain level
      Controlled study
      Juvenile animal
      Nonhuman
      Protein analysis
      Protein depletion
      Protein expression
      Protein function
      Protein localization
      Sequence homology
      Sex difference
      Synaptic transmission
      Western blotting
      Zebra fish
      Analysis of variance
      Animal
      Animal model
      Brain
      Genetics
      Human
      Metabolism
      Physiology
      Sexual characteristics
      Synapse
      Brain
      Carrier Proteins
      SAP90-PSD95 Associated Proteins
      Sex Characteristics
      Synapses
      Synaptophysin
      Permalink
      http://hdl.handle.net/11693/37350
      Published Version (Please cite this version)
      https://doi.org/10.1016/j.neurobiolaging.2017.02.007
      Collections
      • Aysel Sabuncu Brain Research Center (BAM) 201
      • Department of Molecular Biology and Genetics 460
      • Department of Psychology 176
      • Institute of Materials Science and Nanotechnology (UNAM) 1890
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