Site-Specific Fluorescence Polarization for Studying the Disaggregation of α-Synuclein Fibrils by Small Molecules
American Chemical Society
683 - 691
MetadataShow full item record
Please cite this item using this persistent URLhttp://hdl.handle.net/11693/37311
Fibrillar aggregates of the protein α-synuclein (αS) are one of the hallmarks of Parkinson’s disease. Here, we show that measuring the fluorescence polarization (FP) of labels at several sites on αS allows one to monitor changes in the local dynamics of the protein after binding to micelles or vesicles, and during fibril formation. Most significantly, these site-specific FP measurements provide insight into structural remodeling of αS fibrils by small molecules and have the potential for use in moderate-throughput screens to identify small molecules that could be used to treat Parkinson’s disease. © 2016 American Chemical Society.
- Research Paper 7144
Showing items related by title, author, creator and subject.
Protein folding, misfolding and aggregation: The importance of two-electron stabilizing interactions Cieplak A.S. (Public Library of Science, 2017)Proteins associated with neurodegenerative diseases are highly pleiomorphic and may adopt an all-α-helical fold in one environment, assemble into all-β-sheet or collapse into a coil in another, and rapidly polymerize in ...
Novel monoclonal antibodies detect Smad-interacting protein 1 (SIP1) in the cytoplasm of human cells from multiple tumor tissue arrays Oztas, E.; Avci, M.E.; Ozcan, A.; Sayan, A.E.; Tulchinsky, E.; Yagci, T. (2010)Smad-interacting protein 1 (SIP1, also known as ZEB2) represses the transcription of E-cadherin and mediates epithelial-mesenchymal transition in development and tumor metastasis. Due to the lack of human SIP1-specific ...
DNA damage-dependent interaction of the nuclear matrix protein C1D with translin-associated factor X (TRAX) Erdemir, T.; Bilican, B.; Oncel, D.; Goding, C.R.; Yavuzer, U. (2002)The nuclear matrix protein C1D is an activator of the DNA-dependent protein kinase (DNA-PK), which is essential for the repair of DNA double-strand breaks (DSBs) and V(D)J recombination. C1D is phosphorylated very efficiently ...